Recombinant Mouse Thrombopoietin (TPO) (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus oncogene ligand (Mpl-ligand), Megakaryocyte growth and development factor (MGDF), THPO.
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Product Citations
publications
Thrombopoietin_Mouse_Recombinant_BA_010614
Mouse TPO (BL) induces proliferation of M-07e cells.
  • Thrombopoietin_Mouse_Recombinant_BA_010614
    Mouse TPO (BL) induces proliferation of M-07e cells.
Cat # Size Price Quantity Avail. Save
593302 10 µg 148,00€
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593304 25 µg 260,00€
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593306 100 µg 680,00€
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Description

Mouse thrombopoietin (TPO) is a protein consisting of an N-terminus domain with homology to erythropoietin (EPO) and a C-terminus domain with multiple N-linked and O-linked glycosylation sites. TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. It signals through the c-mpl receptor and stimulates the proliferation and maturation of megakaryocytes, hence, TPO plays an important role in regulating the level of circulating platelets in vivo. It has also been shown that TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitors. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes the apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse TPO, amino acids (Ser22-Thr356) (Accession# NP_001166976.1) was expressed in CHO cell line.
Molecular Mass
Predicted molecular mass of approximately 35.5 kD. The protein migrates at about 40 kD in DTT-reducing conditions and about 35 kD in non-reducing condition by SDS-PAGE.The N-terminal amino acid is Ser.
Purity
>90%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg of protein as determine by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Mouse TPO induces proliferation of M-O7e cells with a ED50 < 0.8 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Pariser DN, et al. 2021. J Clin Invest. 131:00:00. PubMed
  2. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  3. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed

Antigen Details

Structure
Monomer
Distribution
TPO is produced by liver and kidney.
Function
TPO regulates megakaryocytopoiesis and thrombopoiesis.
Interaction
Megakaryocytes
Cell Type
Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Avanzi G. et al. 1988. Br. J. Haematol. 69:359.
2. Deutsch V.R. and Tomer A. 2006. Br. J. Haematol. 134:453.
3. Kaushansky K. 2005. J. Clin. Invest. 115:3339.
4. Li J. et al. 1999. Br. J. Haematol. 106:345.
5. Kato T. et al. 1997. Proc. Natl. Acad. Sci. 94:4669.
6. Ehrenreich H. et al. 2005. Proc. Natl. Acad. Sci. 102:862.

Gene ID
21832 View all products for this Gene ID
UniProt
View information about Thrombopoietin on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)

Other Formats

View All Thrombopoietin Reagents Request Custom Conjugation
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Recombinant Human IL-11 (carrier-free) BA
Recombinant Mouse CCL7 (MCP-3) (carrier-free) BA
Recombinant Mouse CCL9 (MIP-1γ) (carrier-free) BA
Recombinant Mouse CCL21 (6CKine) (carrier-free) BA
Recombinant Mouse Thrombopoietin (TPO) (Animal-Free) BA
Recombinant Mouse IL-3 (Animal-Free) BA
Recombinant Mouse IL-4 (Animal-Free) BA
Recombinant Human IL-21 (carrier-free) BA
Recombinant Mouse CXCL13 (carrier-free) BA
LEGEND MAX™ Human sICAM-1/CD54 ELISA Kit with Pre-coated Plates ELISA
Stop Solution for TMB Substrate ELISA
Zombie Aqua™ Fixable Viability Kit FC, ICFC, ICC
LEGEND MAX™ Human CXCL10 (IP-10) ELISA Kit ELISA
Recombinant Mouse CXCL7 (NAP-2) (carrier-free) BA
Recombinant Mouse CXCL3 (carrier-free) BA
Recombinant Mouse CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL23 (MPIF-1) (Arg46-Asn120) (carrier-free) BA
Recombinant Human Erythropoietin (EPO) (carrier-free) BA
Recombinant Mouse IL-25 (IL-17E) (insect expressed, carrier-free) BA
Recombinant Human FASL (TNFSF6) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) ELISA
Recombinant Mouse CXCL14 (BRAK) (carrier-free) BA
Recombinant Human CXCL14 (BRAK) (carrier-free) BA
Recombinant Mouse Erythropoietin (EPO) (carrier-free) BA
Recombinant Human CXCL12 (SDF-1β) (carrier free) BA
Recombinant Mouse CCL19 (MIP-3β) (carrier-free) BA
Recombinant Mouse CCL12 (MCP-5) (carrier-free) BA
Zombie NIR™ Fixable Viability Kit FC, ICFC
Recombinant Human VEGF-C (carrier-free) BA
Recombinant Mouse NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Human NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Mouse IGF-II (carrier-free) BA
Purified Annexin V FC
Recombinant Mouse CXCL12 (SDF-1β) (carrier-free) BA
Recombinant Human TGF-α (carrier-free) BA
Recombinant Rat CCL20 (MIP-3α) (carrier-free) BA
LEGEND MAX™ Human CCL17 (TARC) ELISA Kit ELISA
LEGEND MAX™ Human CCL5 (RANTES) ELISA Kit ELISA
Recombinant Mouse CXCL4 (PF-4) (carrier-free) BA
Accutase® Cell Detachment Solution FC
Recombinant Human IGF-II (carrier-free) BA
Recombinant Human IGF-I (carrier-free) BA
Recombinant Human TRANCE (RANKL) (carrier-free) BA
Recombinant Mouse BAFF (carrier-free) BA
Recombinant Human APRIL (TNFSF13) (ELISA Std.) ELISA
Recombinant Human IL-15 (ELISA Std.) ELISA
Recombinant Mouse IGF-I (carrier-free) BA
Recombinant Human sTNF-RII (TNFRSF1B) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL17 (TARC) ELISA
Recombinant Mouse CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Human CD40L (TNFSF5) (carrier-free) BA
Recombinant Human CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Mouse TNF-α (Animal-Free) BA
Recombinant Human Activin A (carrier-free) BA
Recombinant Human FGF-4 (carrier-free) BA
Recombinant Human IL-18 (carrier-free) BA
Recombinant Rat GM-CSF (carrier-free) BA
Recombinant Human IFN-α2 (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL5 (RANTES) ELISA
ELISA MAX™ Deluxe Set Human CXCL5 ELISA
LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit ELISA
ELISA MAX™ Deluxe Set Human CCL28 ELISA
Recombinant Rat Erythropoietin (EPO) (carrier-free) BA
Recombinant Human PCSK9 (carrier-free) BA
Recombinant Human CCL17 (TARC) (ELISA Std.) ELISA
Recombinant Human TIMP-1 (carrier-free) BA
Recombinant Human Arginase I (carrier-free) BA
Recombinant Human MMP-1 (carrier-free) BA
Recombinant Human CD14 (carrier-free) BA
Recombinant Human CTLA-4 (ELISA Std.) ELISA
Recombinant Mouse IL-34 (ELISA Std.) ELISA
Recombinant Human IL-34 (ELISA Std.) ELISA
Recombinant Human Latent TGF-β (ELISA Std.) ELISA
Recombinant Human Mesothelin-Fc Chimera (carrier-free) BA
Recombinant Human TIMP-2 (carrier-free) BA
Recombinant Mouse TIMP-1 (carrier-free) BA
Brilliant Violet 421™ Annexin V FC
Alexa Fluor® 488 Streptavidin FC, ICFC
Alexa Fluor® 647 Streptavidin FC, ICFC
Recombinant Mouse CCL3 (MIP-1α) (carrier-free) BA
Recombinant Human LIF (carrier-free) BA
Recombinant Mouse Mesothelin (carrier-free) BA
Recombinant Rat IFN-γ (ELISA Std.) ELISA
Recombinant Mouse IL-17A/F Heterodimer (ELISA Std.) ELISA
Recombinant Human TGF-β1 (ELISA Std.) ELISA
Zombie Violet™ Fixable Viability Kit FC, ICFC, ICC
Zombie Green™ Fixable Viability Kit FC, ICFC, ICC
Zombie Red™ Fixable Viability Kit FC, ICFC, ICC
Zombie UV™ Fixable Viability Kit FC, ICFC
CFSE Cell Division Tracker Kit ICFC, in-vivo Cell Tracking
LEGEND MAX™ Human Adiponectin (Acrp30) ELISA Kit ELISA
Bromodeoxyuridine (BrdU) FC, ICFC
Recombinant Mouse CCL5 (RANTES) (carrier-free) BA
Recombinant Mouse IFN-β (ELISA Std.) ELISA
Recombinant Human IL-9 (carrier-free) BA
Recombinant Mouse Latent TGF-β (ELISA Std.) ELISA
Recombinant Human MMP-3 (carrier-free) BA
APC Streptavidin (High Concentration) FC, ICFC
Recombinant Human CCL2 (MCP-1) (Animal-Free) BA
Recombinant Human VEGFR2-Fc Chimera (carrier-free) BA
Recombinant Human NT-4 (Animal-Free) BA
Cell Activation Cocktail (without Brefeldin A) Activ, FC, ICFC
Recombinant Human RBP4 (carrier-free) BA
Recombinant Rat IFN-γ (carrier-free) BA
Recombinant Human ICOS-Fc Chimera (carrier-free) BA
Cell Activation Cocktail (with Brefeldin A) Activ, FC, ICFC
Recombinant Mouse ICOS-Fc Chimera (carrier-free) BA
Recombinant Mouse VEGF-164 (Animal-Free) BA
Recombinant Human NT-3 (carrier-free) BA
Recombinant Human NT-4 (carrier-free) BA
Recombinant Human FGF-1-acidic (carrier-free) BA
Nunc™ MaxiSorp™ ELISA Plates, Uncoated ELISA
Plate Sealers ELISA
DRAQ7™ FC, ICFC
Recombinant Human WISP-1 (carrier-free) BA
Recombinant Human WNT-7a (carrier-free) BA
Recombinant Human BMP-4 (carrier-free) BA
Go To Top Version: 2    Revision Date: 01/20/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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