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RBC Lysis/Fixation Solution (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysing solution
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Product Citations
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RBClysis_422401_1_092111
Human peripheral blood cells were stained with anti-human CD3 PerCP/Cy5.5 and anti-human CD4 FITC; red blood cells were lysed with BioLegend RBC Lysis/Fixation Solution.
  • RBClysis_422401_1_092111
    Human peripheral blood cells were stained with anti-human CD3 PerCP/Cy5.5 and anti-human CD4 FITC; red blood cells were lysed with BioLegend RBC Lysis/Fixation Solution.
  • RBClysis_422401_2_092111
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422401 50 mL 113€
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Description

BioLegend RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation  of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H20 prior to use.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1 - 7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage


Whole Blood Lyse/Wash Procedure:

1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20.
2. Mix 100 µl of whole blood with fluorochrome-conjugated antibody.
3. Incubate at room temperature, in the dark, for 20 minutes.
4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
5. Incubate at room temperature, in the dark, for 15 minutes.
6. Centrifuge at 350Xg for five minutes.  Discard supernatant.
7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201).
8. Centrifuge at 350Xg for five minutes. Discard supernatant.
9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer.
10. Analyze cells immediately by flow cytometry or store at 4°C, in the dark, for no longer than 12 hours.

Whole Blood Lyse/No Wash Procedure:

1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20.
2. Mix 50 µl of whole blood with fluorochrome-conjugated antibody.
3. Incubate at room temperature, in the dark, for 20 minutes.
4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
5. Incubate at room temperature, in the dark, for 15 minutes.
6. Analyze samples by flow cytometry.

Application Notes

The 1X Lysis/Fixation solution can be used within 1 month from the date of preparation. It can be stored at room temperature or between 2°C and 8°C.

Application References
  1. Horvath P, et al. 2012. J Infect Dis. PubMed
Product Citations
  1. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  2. Curry A, et al. 2017. PLoS One. 12(2):e0171586. PubMed
  3. Nerdal P, et al. 2016. J Immunol. 197: 3059 - 3068. PubMed
  4. Lee J, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.014. PubMed
  5. Horváth P, et al. 2012. J Infect Dis. 205:923. PubMed
  6. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  7. Camponova P, et al. 2017. Can J Physiol Pharmacol. 10.1139/cjpp-2016-0710. PubMed
  8. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  9. Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed

Antigen Details

Gene ID
NA

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Go To Top Version: 2    Revision Date: 10/17/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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