- Poly5232 (See other available formats)
- Regulatory Status
- Other Names
- Leukemia Inhibitory factor, HILDA, CDF, DIA, MLPLI
- Goat Polyclonal Ig
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- Product Citations
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Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.Product Details
- Antibody Type
- Host Species
- Recombinant Mouse LIF
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.5 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of LIF recombinant protein ranging from 1200 to 18.75 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The pure Poly5232 (Cat. No. 523201) antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5232 antibody (Cat. No. 523204) as the detection antibody and Recombinant Recombinant Mouse LIF (ELISA Std.) (Cat. No. 554009).
- Product Citations
AB_2814445 (BioLegend Cat. No. 523201)
Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells
- Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells
- Embryonic stem cells
- Gene ID
- 16878 View all products for this Gene ID
- View information about LIF on UniProt.org
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