Loading...

PerCP anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M1slash70_PerCP_102110
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP (filled histogram) or rat IgG2b PerCP isotype control (open histogram) (gated on total cell population).
  • M1slash70_PerCP_102110
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP (filled histogram) or rat IgG2b PerCP isotype control (open histogram) (gated on total cell population).
Compare all formats See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
101229 25 µg 85€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101230 100 µg 231€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. De Vries LCS, et al. 2021. J Crohns Colitis. 15:617. PubMed
  2. Liang Y, et al. 2021. Cell Death Differ. 28:2728. PubMed
  3. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  4. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  5. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  6. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  7. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  8. Pott J et al. 2018. Cell host & microbe. 23(2):191-202 . PubMed
  9. Neumann AM et al. 2017. PloS one. 12(11):e0187330 . PubMed
  10. Wang S, et al. 2022. Front Immunol. 13:869061. PubMed
  11. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  12. Kaczmarek–Hájek K, et al. 2018. Elife. 7:e36217. PubMed
  13. Lyons J, et al. 2016. PLoS One. 11: 0149069. PubMed
  14. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  15. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  16. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  17. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  18. Rostoker R, et al. 2013. Prostaglandins Other Lipid Mediat. 107:85. PubMed
  19. Ben-Shaanan T, et al. 2016. Nat Med. 10.1038/nm.4133. PubMed
  20. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  21. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
  22. Yu CR, et al. 2022. Int J Mol Sci. 23:. PubMed
  23. Alexaki V, et al. 2017. Mol Psychiatry. 10.1038/mp.2017.167. PubMed
  24. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
  25. Yang P, et al. 2022. Nat Commun. 13:5782. PubMed
  26. Schleicher R, et al. 2015. Blood. 126: 1483-1493. PubMed
  27. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  28. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  29. Lee S et al. 2014. The Journal of Neuroscience. 34(37):12538-46 . PubMed
  30. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  31. Yazlovitskaya EM, et al. 2021. J Cell Sci. 134:. PubMed
  32. Yao S, et al. 2021. FEBS Open Bio. 11:1607. PubMed
  33. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  34. Guo J, et al. 2020. J Immunol. 205:2916. PubMed
  35. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  36. Pashover-Schallinger E, et al. 2012. FASEB J. 26:3891. PubMed
  37. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  38. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  39. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  40. Cheng Y, et al. 2022. iScience. 25:103979. PubMed
  41. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  42. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  43. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  44. Zager A, et al. 2017. Progress in Neuro-Psychopharmacology and Biological Psychiatry. 10.1016/j.pnpbp.2017.05.003. PubMed
  45. Li C, et al. 2018. Cell. 174:285. PubMed
  46. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  47. Lv JJ, et al. 2021. Front Cell Dev Biol. 8:609090. PubMed
  48. Deng P, et al. 2021. Cell Stem Cell. 28(6):1057-1073.e7. PubMed
  49. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  50. Cederquist C, et al. 2017. Mol Metab. 6(1):125-137. PubMed
  51. Hwang BJ, et al. 2019. Oncogene. 38:7491. PubMed
  52. Varvel N, et al. 2016. Proc Natl Acad Sci U S A. 113: E5665 - E5674. PubMed
  53. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  54. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  55. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  56. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  57. Legrain S, et al. 2015. J Immunol. 195: 4171 - 4175. PubMed
  58. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  59. Bandrowski AE et al. 2016. Neuron. 90(3):434-6 . PubMed
  60. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  61. Martinez L, et al. 2020. J Immunol. 205:2545. PubMed
  62. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
RRID
AB_2129375 (BioLegend Cat. No. 101229)
AB_2129374 (BioLegend Cat. No. 101230)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC,IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 806 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 780 anti-mouse/human CD11b M1/70 FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
Spark PLUS UV™ 395 anti-mouse/human CD11b M1/70 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account