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PE/Cyanine7 anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
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Product Citations
publications
GL-1_PECy7_090707
C57BL/6 mouse splenocytes stained with GL-1 PE/Cyanine7
  • GL-1_PECy7_090707
    C57BL/6 mouse splenocytes stained with GL-1 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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105013 25 µg 76€
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105014 100 µg 226€
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Kuo PC, et al. 2021. Brain Commun. 3:fcab187. PubMed
  2. Zhang Z, et al. 2022. Front Pharmacol. 13:906625. PubMed
  3. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  4. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  5. Amir M, et al. 2020. Biochem Biophys Res Commun. 527:1000. PubMed
  6. Limon JJ et al. 2019. Cell host & microbe. 25(3):377-388 . PubMed
  7. Kim CC et al. 2019. Cell Rep. 27(5):1446-1460 . PubMed
  8. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  9. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  10. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  11. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  12. Xu AQ, et al. 2020. Elife. 9:00. PubMed
  13. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  14. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  15. Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
  16. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  17. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  18. Zhang X, et al. 2022. Cells. 11:. PubMed
  19. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  20. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  21. Li Z, et al. 2022. J Exp Clin Cancer Res. 41:74. PubMed
  22. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  23. Polavaram NS, et al. 2021. Bone Res. 9:24. PubMed
  24. Nguyen TKT, et al. 2020. Am J Pathol. 286:190. PubMed
  25. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  26. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  27. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  28. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  29. Guo L, et al. 2021. Vaccines (Basel). 9:. PubMed
  30. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  31. Trabucco S, Gerstein R, Zhang H 2016. J Immunol. 197: 1699 - 1707. PubMed
  32. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  33. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  34. Fox C, et al. 2016. J Control Release. 244:98-107. PubMed
  35. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  36. Lee W, et al. 2021. PLoS Pathog. 17:e1009168. PubMed
  37. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  38. Kelly SH, et al. 2021. ACS Biomater Sci Eng. 7:1876. PubMed
  39. Tukaramrao DB, et al. 2021. Cancers (Basel). 13:. PubMed
  40. Yang X, et al. 2022. Front Immunol. 13:856230. PubMed
  41. Toki S, et al. 2013. Nanomedicine. 9:1235. PubMed
  42. Ma Y, et al. 2022. Front Immunol. 13:816952. PubMed
  43. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  44. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
  45. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  46. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  47. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
  48. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
  49. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  50. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  51. Smith LK, et al. 2021. Elife. 10:. PubMed
  52. Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed
  53. Perper SJ, et al. 2019. J Immunol. 203:58. PubMed
  54. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  55. Lu H, et al. 2011. Toxicol Appl Pharmacol. 255:251. PubMed
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  57. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_439782 (BioLegend Cat. No. 105013)
AB_439783 (BioLegend Cat. No. 105014)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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