PE anti-human Perforin Antibody

Pricing & Availability
Clone
dG9 (See other available formats)
Regulatory Status
RUO
Other Names
PRF1, P1, PFP, HPLH2
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
dG9_PE_072607
Human peripheral blood lymphocytes intracellularly stained with dG9 PE
  • dG9_PE_072607
    Human peripheral blood lymphocytes intracellularly stained with dG9 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
308105 25 tests 97€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
308106 100 tests 220€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified granules from the human lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.


Does not cross-react with mouse1.

Application References
  1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
  2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
  3. Watanabe N, et al. 1997. Blood 90:3662.
  4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
  5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
  6. Chen H, et al. 2005. J. Immunol. 175:591.
  7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
  8. Wood SM, et al. 2009. Blood 114:4117. PubMed
  9. Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
Product Citations
  1. Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed
  2. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  3. Lettau M, et al. 2018. Int Immunol. 30:215. PubMed
  4. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  5. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  6. Kuttruff S, et al. 2009. Blood. 113:358. PubMed
  7. Bertino P, et al. 2014. Vaccine. 32:1670. PubMed
RRID
AB_314703 (BioLegend Cat. No. 308105)
AB_314704 (BioLegend Cat. No. 308106)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 03/14/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human Perforin

  • PE anti-human Perforin

  • Purified anti-human Perforin

  • Alexa Fluor® 488 anti-human Perforin

  • Alexa Fluor® 647 anti-human Perforin

  • APC anti-human Perforin

  • Pacific Blue™ anti-human Perforin

  • PerCP/Cyanine5.5 anti-human Perforin

  • Brilliant Violet 510™ anti-human Perforin

  • Brilliant Violet 421™ anti-human Perforin

  • Alexa Fluor® 594 anti-human Perforin

  • APC/Cyanine7 anti-human Perforin

  • PE/Cyanine7 anti-human Perforin

  • Brilliant Violet 711™ anti-human Perforin

  • PE/Dazzle™ 594 anti-human Perforin

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account