ELISA MAX™ Deluxe Set Mouse TNF-α

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mouse tnf-a_122109
  • mouse tnf-a_122109
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430904 5 plates 229€
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430915 10 Plates 440€
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430916 20 Plates 704€
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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  2. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  3. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  4. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  5. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  6. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  7. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  8. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  9. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  10. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  11. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  12. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  13. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  14. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  15. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  16. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  17. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  18. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  19. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  20. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  21. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  22. Cobb C 2010. J Immunol. 184:3789. PubMed
  23. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  24. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  25. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  26. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  27. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  28. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  29. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  30. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  31. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  32. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  33. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  34. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  35. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  36. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  37. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  38. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  39. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  40. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  41. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  42. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
21926 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12/14/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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