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HRP Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
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406401 500 µL 113€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

WB - Quality tested
ELISA, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed
  2. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  3. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  4. Miki H, et al. 2020. J Immunol. 204:753. PubMed
  5. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  6. Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed
  7. Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed
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  9. Lam DD, et al. 2022. J Neurosci. 42:1557. PubMed
  10. Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed
  11. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  12. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  13. Xu H, et al. 2021. Microb Cell Fact. 20:115. PubMed
  14. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  15. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  16. Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed
  17. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  18. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  19. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  20. Saiz ML, et al. 2021. Clin Epigenetics. 13:187. PubMed
  21. Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed
  22. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  23. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  24. Ashley CL, et al. 2019. Viruses. 11:3. PubMed
  25. Chan TC, et al. 2021. Clin Transl Med. 11:e674. PubMed
  26. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  27. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  28. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  29. Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed
  30. Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed
  31. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  32. Yu C, et al. 2021. Front Genet. 12:689902. PubMed
  33. Moffat J, et al. 2013. Vaccine. 31:2310. PubMed
  34. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  35. Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
  36. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  37. Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed
  38. Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2099368 (BioLegend Cat. No. 406401)

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Go To Top Version: 4    Revision Date: 12/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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