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GMP Recombinant Human TNF-α (carrier-free)

Pricing & Availability
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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GMP recombinant human TNF-α induces cytotoxicity of L929 cells in a dose-dependent manner with an ED50 range of 0.01 - 0.085 ng/mL.
  • Human_TNF-a_CF_GMP-RECOM_022221.png
    GMP recombinant human TNF-α induces cytotoxicity of L929 cells in a dose-dependent manner with an ED50 range of 0.01 - 0.085 ng/mL.
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570114 25 µg 254€
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570116 100 µg 756€
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Description

TNF-α was originally described as an endotoxin-induced, macrophage-derived factor that promotes hemorrhagic necrosis of solid tumors and the cachexia of chronic infections. TNF-α has also been implicated in a range of inflammatory, infectious, and malignant disorders. At the cellular level, TNF-α modulates a broad spectrum of responses including inflammation, immunoregulation, proliferation, apoptosis, and antiviral activity. In bone, the cytokine inhibits extracellular matrix deposition, stimulates matrix metalloprotease synthesis, and enhances production of osteoclastogenic cytokines such as M-CSF and RANKL. Chronic exposure to TNF-α in vivo increases osteoclastogenesis through two distinct mechanisms. TNF-α first affects osteoclastogenesis at the osteoclast precursor stage in the bone marrow by priming these cells to differentiate into cFms+/CD11b+/RANK+/- osteoclast progenitors via a RANKL/RANK independent mechanism. These osteoclast precursors then enter the blood and peripheral tissues where they differentiate into mature osteoclasts in the presence of RANKL, and this process is accelerated by TNF. The role of TNF at this later stage of osteoclast differentiation is RANKL/RANK dependent. Importantly, TNF-α promotes bone resorption both in vitro and in vivo by enhancing the proliferation and differentiation of osteoclast precursors.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TNF-α, amino acids Val77-Leu233 (Accession# NM_000594), was expressed in E. coli.
Molecular Mass
The 157 amino acid recombinant protein has a predicted molecular mass of 17.35 kD. The DTT-reduced protein and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Val.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.1 µm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.01 - 0.085 ng/mL as determined by the dose-dependent cytotoxicity of L929 cells stimulated with actinomycin D.
Application

Bioassay
Cell Culture
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures.  Our processes include:

  • Batch-to-batch consistency   
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of releaed products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
TNF superfamily
Distribution

It is produced primarily by macrophages, and it is also expressed by activated T cells, B cells, NK cells, and neutrophils.

Function
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
Interaction
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Bioactivity
Measured by its ability to induce cytotoxicity of L929 cells stimulated with actinomycin D
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Boyce BF, et al. 2005. J Med 54:127-131.
  2. Lam J, et al. 2000. J Clin Invest 106:1481-1488.
  3. Udagawa N, et al. 2002. Arthritis Res 4:281-289.
  4. Kwon J, et al. 1993. Gene 132:227-236.
  5. Harth S, et al. 2019. MAbs. 11:178.
  6. de Oliveira Mann CC, et al. 2019. Cell Rep. 27:1165.
  7. Anderson NR, et al. 2019. Cell Adh Migr. 13:163.
  8. Zhichao Fan, et al. 2019. Cell Rep. 26(1):119-130.
  9. VanDussen KL, et al. 2019. Stem Cell Res. 37:101430.
  10. Hurrell BP, et al. 2019. Cell Rep. 29:4509.
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org
Go To Top Version: 1    Revision Date: 02/22/2021

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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