FITC anti-mouse Ly-6G Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Excitation Laser

Blue Laser (488 nm)

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
2. Campbell IK, Leong D 2016. J Immunol. 197(11):4392-4402. PubMed
3. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
4. Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed
5. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
6. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
7. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
8. Golden GJ, et al. 2021. MBio. 12:e0118121. PubMed
9. He R, et al. 2021. J Immunol. 206:2353. PubMed
10. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
11. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
12. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
13. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
14. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
15. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
16. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
17. Long M, et al. 2017. J Immunol. 198(2):862-872. PubMed
18. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
19. Mandraju R, et al. 2014. J Immunol. 192:4303. PubMed
20. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
21. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
22. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
23. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
24. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
25. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
26. Patsalos A, et al. 2019. J Immunol. 203:1532. PubMed
27. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
28. Han J, et al. 2022. FEBS J. 289:417. PubMed
29. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
30. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
31. Torre S, et al. 2016. Nat Immunol. 10.1038/ni.3581. PubMed
32. Montufar-Solis D, Klein J 2016. PLoS One. 11: 014611. PubMed
33. Wang C, et al. 2015. Sci Rep. 5: 14124. PubMed
34. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
35. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
36. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
37. Bae SH, et al. 2021. Theranostics. 11:2522. PubMed
38. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
39. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
40. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
41. Guesdon W, et al. 2015. J Infect Dis. 212: 1332 - 1340. PubMed
42. Strother R, et al. 2016. J Immunol. 197(11):4301-4311. PubMed
43. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
44. Han YH, et al. 2021. Science. 373:. PubMed
45. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
46. Diao N, et al. 2016. Sci Rep. 6:34672. PubMed
47. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
48. Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
49. Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed
50. Saika A, et al. 2020. FASEB Bioadv. 2:59. PubMed
51. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
52. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
53. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
54. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
55. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
56. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
57. Wu X, et al. 2021. Elife. 10:. PubMed
58. Opzoomer JW, et al. 2021. Sci Adv. 7:eabg9518. PubMed
59. Abad C, et al. 2014. J Leukoc Biol. 95:357. PubMed
60. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
61. Liu Z, et al. 2016. J Biol Chem. 291: 1441 - 1447. PubMed
62. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
63. Na YR, et al. 2020. Cell Rep. 107643:31. PubMed
64. Wang L, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01754. PubMed
65. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
66. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
67. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
68. Jacome-Galarza C, et al. 2011. J Bone Miner Res. 26:1207. PubMed
69. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
70. Jin RM, et al. 2017. J Immunol. 198:352. PubMed
71. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
72. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
73. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
74. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
75. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
76. Geng S, et al. 2021. JCI Insight. 6:. PubMed
77. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
78. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
79. Chen M, et al. 2021. Oncol Lett. 21:435. PubMed
80. Minogue A, et al. 2014. Neurobiol Aging. 35:1442. PubMed
81. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
82. Zheng J, et al. 2020. bioRxiv. . PubMed
83. Huixin Xu et al. 2018. EMBO molecular medicine. 10(9) pii: e8931. PubMed
84. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
85. Zuo W, et al. 2022. Cell Death Discov. 8:257. PubMed
86. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
87. Gousopoulos E, et al. 2016. Am J Pathol. 186:2193-2203. PubMed
88. Radjabova V, et al. 2015. J Immunol. 195: 3149 - 3159. PubMed
89. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
90. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
91. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
92. Roy K, et al. 2019. Immunity. 50:616. PubMed
93. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
94. Christ A, et al. 2018. Cell. 172:162. PubMed
95. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
96. Flögel U, et al. 2021. Nat Commun. 12:5847. PubMed
97. Wu M, et al. 2013. Proc Natl Acad Sci U S A. 110:9439. PubMed
98. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
99. Ericson J, et al. 2014. PLoS One. 9:108553. PubMed
100. Shih Y, et al. 2015. JPEN J Parenter Enteral Nutr. . PubMed
101. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
102. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
103. He J, et al. 2019. J Clin Invest. 130. PubMed
104. Yu L, et al. 2019. Elife. 8:e46677. PubMed
105. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
106. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
107. Zheng J, et al. 2020. Nature. . PubMed
108. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
109. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
110. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
111. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
112. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
113. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
114. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
115. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
116. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
117. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
118. Simpson K, et al. 2012. J Immunol. 189:5533. PubMed
119. Giampazolias E, et al. 2021. Cell. . PubMed
120. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
121. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
122. Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed
123. Rebecca S Dickinson et al. 2017. Wellcome open research. 0.155555556 . PubMed
124. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
125. Qin W, et al. 2022. Bio Protoc. 12:e4287. PubMed
126. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
127. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
128. Taya M, et al. 2020. Endocr Relat Cancer. 27:261. PubMed
129. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
130. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
131. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
132. Li YT, et al. 2019. Elife. 8:e47642. PubMed
133. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
134. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
135. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
136. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
137. Makino A, et al. 2021. iScience. 24:103201. PubMed
138. Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed
139. Njock MS, et al. 2022. J Extracell Vesicles. 11:e12228. PubMed
140. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
141. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
142. Gamradt P, et al. 2016. PLoS Pathog. 12:e1006032. PubMed
143. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
144. Thomas DG et al. 2018. Cell reports. 25(13):3774-3785 . PubMed
145. Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed
146. Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed
147. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
148. van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed
149. Gu H, et al. 2021. Elife. 10:. PubMed
150. Issah Y, et al. 2021. Elife. 10:. PubMed
151. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
152. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
153. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
154. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
155. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
156. Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed
157. Danzer H, et al. 2020. Elife. 9:00. PubMed
158. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
159. Smith LK, et al. 2021. Elife. 10:. PubMed
160. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
161. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
162. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
163. Xu Y, et al. 2014. Proc Natl Acad Sci U S A. 111:6371. PubMed
164. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
165. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
166. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
167. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
168. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
169. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
170. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
171. Zhang H, et al. 2022. Commun Biol. 5:916. PubMed
172. Stadtmann A, et al. 2015. J Immunol. 195: 1152-1161. PubMed
173. Jia H, et al. 2016. J Immunol. 197: 859 - 871. PubMed
174. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
175. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
176. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
177. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
178. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
179. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
180. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
181. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
182. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
183. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
184. Chuang LT, et al. 2021. Molecules. 26:. PubMed
185. Singh N, et al. 2016. Dis Model Mech. 9: 885 - 897. PubMed
186. Cojohari O, Peppenelli M, Chan G 2016. Blood. 128: 529 - 541. PubMed
187. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed

RRID

AB_1236488 (BioLegend Cat. No. 127605)
AB_1236494 (BioLegend Cat. No. 127606)

Antigen Details

Structure

A 21-35 kD GPI-anchorded membrane protein

Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type

Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID

546644 View all products for this Gene ID

UniProt

View information about Ly-6G on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis