Biotin anti-mouse IL-17F Antibody

Pricing & Availability
Clone
8F5.1A9 (See other available formats)
Other Names
Interleukin-17F, ML-1
Isotype
Mouse IgG1, κ
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517105 50 µg 120€
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Description

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Overexpression of the IL-17F gene in the airways of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo.  P38 MAPK, ERK1/2, Act1 (NF-kB activator protein 1), and TRAF6 are involved in IL-17F signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-17F-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1.0-4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-17A/F recombinant protein ranging from 500 to 7.8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated 8F5.1A9 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10.1 (Cat. No. 506901 or 506906) antibody as the capture antibody and recombinant mouse IL-17A/F (Cat. No. 580809) as the standard.

Flow Cytometry: The fluorochrome-labeled 8F5.1A9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17F-producing cells within mixed cell populations.

Note: For testing mouse IL-17F in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 436107 & 436108) are specially developed and recommended.

RRID
AB_2562987 (BioLegend Cat. No. 517105)

Antigen Details

Structure
Forms a 37 kD homodimer or heterodimer with IL-17A, a IL-17 family member.
Distribution
Th17 cells, mast cells, basophils, and epithelial cells.
Function
An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor
IL-17R (CD217)
Cell Type
Th17, Mast cells, Basophils, Epithelial cells, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.

Gene ID
257630 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 01/23/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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