Biotin anti-human PlGF Antibody

Pricing & Availability
Clone
24G3 (See other available formats)
Regulatory Status
RUO
Other Names
PlGF, PLGF, Placental Growth Factor
Isotype
Mouse IgG2a
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Avail. Save
534904 100 µg 180,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Placental growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors. Four alternatively-spliced isoforms have been identified: PlGF-1, PlGF-2, PlGF-3, and PlGF-4. All isoforms are expressed in placental tissues, with PlGF-2 being expressed specifically during early placental development. PlGF-1 and PlGF-3 are considered diffusible isoforms, while PlGF-2 and PlGF-4 each contain a unique heparin binding domain that may increase associations with cell membranes. In addition to placental tissues, PlGF is expressed in the microvasculature, bone marrow, umbilical vein endothelia, uterine keratinocytes, and NK cells. PlGF binds to both VEGF-R1 and neuropilin, but not VEGF-R2 and induces monocyte activation, migration, inflammatory cytokine production. PlGF plays a key role in maternal vascular function during pregnancy, with circulating levels reaching a peak mid-gestation. It can be used as a marker for pregnancy-associated hypertensive disorders such as pre-eclampsia.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA-D - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.1 - 4.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotin 24G3 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with pure 13C8 antibody (Cat. No. 534802) as the capture antibody.

Product Citations
RRID
AB_2894609 (BioLegend Cat. No. 534904)

Antigen Details

Structure
Homodimer
Distribution

Vascular endothelial cells, smooth muscle cells, keratinocytes, hematopoitic cells, retinal pigment epithelial cells, tumor cells, and cerebellar stroma cells.

Function
PlGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PlGF.
Interaction
Endothelial cells, Macrophages, and trophoblats.
Ligand/Receptor
VEGFR1 (FLT-1), and neuropilin 1 (Nrp1)
Cell Type
Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors
Antigen References
  1. Maglione D, et al. 1991. Proc Natl Acad Sci. USA 88:9267.
  2. Carmeliet P, et al. 2001. Nat Med. 7:575.
  3. Casalou C, et al. 2007. Leukemia. 21:1590.
  4. Marcellini M, et al. 2006. Am J Pathol. 169:643.
  5. Yao J, et al. 2011. Proc Natl Acad Sci USA. 108:11590.
  6. Cai J, et al. 2011. PLoS One. 6:e18076.
  7. Sibiude J, et al. 2012. PLoS One. 7:e50208.
  8. Snuderl M, et al. 2013. Cell. 152:1065.
Gene ID
5228 View all products for this Gene ID
UniProt
View information about PlGF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/14/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account