Biotin anti-human LIF Antibody

Pricing & Availability
M017C3 (See other available formats)
Regulatory Status
Other Names
Leukemia Inhibitory Factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
Mouse IgG1, κ
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530301 50 µg 132€
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Human LIF stands for Leukemia Inhibitory Factor. It belongs to the IL-6 family of cytokines, and is a variably glycosylated, secreted protein with a mass of 38 – 67 kD. The mature form has 180 amino acids and contains six conserved cysteines, which form three intramolecular disulfide bonds. The protein itself forms a four-helix bundle fold. Human LIF shares 78% amino acid identity with mouse LIF.

LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia. It often has contradictory effects within different contexts, either stimulating cell proliferation, or inducing differentiation of mouse M1 leukemic myeloid cell line into macrophages. With cultured embryonic stem cells, LIF has been an important laboratory tool to help maintain stem cell pluripotency for cell cultures. It is involved in the mechanisms of embryonic implantation, inflammatory disorders such as arthritis, immunomodulation, carcinogenesis, and neoplasia-associated cachexia.

LIF binds to both LIF Rα and gp130 on the cell surface to form a heterotrimeric complex, which initiates cell signaling through the JAK-STAT pathway. LIF can also activate the MAPK, PI3K, and p53 signaling pathways. Soluble forms of both binding partners do exist in circulation and potentially serve to further regulate LIF action.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 2.0 µg/ml of the detection antibody was utilized to generate the example standard curve for human LIF. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: To measure human LIF, this antibody can be used as a detection antibody in a sandwich ELISA format and paired with the purified clone Poly5233 antibody as the capture antibody. Recombinant Human LIF (ELISA Std.) can be used as the protein standard.

Note: For testing human LIF in serum, plasma, or supernatant, BioLegend’s LEGEND MAX™ Human LIF ELISA Kit is specially developed and recommended.

AB_2566783 (BioLegend Cat. No. 530301)

Antigen Details

Four-helix bundle fold.
Expressed in the trophectoderm of the developing embryo and activated CD4+ T cells.
LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia.
Embryonic stem cells.
LIF Rα, gp130
Cell Type
T cells
Biology Area
Molecular Family
Antigen References

1. Metcalf D. 1991. Int. J. Cell Cloning 2:95-108.
2. Zhang JG, et al. 1998. J. Biol. Chem. 17:10798.
3. Kamohara H, et al. 2007. Int. J. Oncol. 4:977.
4. Marshall MK, et al. 1994. Endocrinology 135:141.
5. Vogiagis D, Salamonsen LA. 1999. J. Endocrinol. 160:181.
6. Zouein FA, et al. 2013. Eur. Cytokine Netw. 24:11.
7. Mathieu ME, et al. 2012. Stem Cell Rev. 8:1.
8. Hu W, et al. 2007. Nature 450:721.
9. Trouillas M, et al. 2009. Eur. Cytokine Netw. 20:51.
10. Metcalfe SM. 2011. Genes Immun. 3:157.

Gene ID
3976 View all products for this Gene ID
View information about LIF on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 01/18/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Biotin anti-human LIF


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