APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A019D5_APCFire810_CD127_Antibody_102221
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD127 (IL-7Rα) (clone A019D5) APC/Fire™ 810 (left) or mouse IgG1, ĸ APC/Fire™ 810 isotype control (right).
  • A019D5_APCFire810_CD127_Antibody_102221
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD127 (IL-7Rα) (clone A019D5) APC/Fire™ 810 (left) or mouse IgG1, ĸ APC/Fire™ 810 isotype control (right).
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351373 25 tests 146€
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351374 100 tests 344€
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
RRID
AB_2904371 (BioLegend Cat. No. 351373)
AB_2904371 (BioLegend Cat. No. 351374)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD127 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD127 (IL-7Rα) A019D5 FC
PE anti-human CD127 (IL-7Rα) A019D5 FC
Pacific Blue™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα) A019D5 FC
FITC anti-human CD127 (IL-7Rα) A019D5 FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα) A019D5 FC
APC anti-human CD127 (IL-7Rα) A019D5 FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα) A019D5 FC
PE/Cyanine5 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα) A019D5 FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα) A019D5 FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready) A019D5 FC, CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα) A019D5 FC
Biotin anti-human CD127 (IL-7Rα) A019D5 FC
APC/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα) A019D5 FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα) A019D5 PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα) A019D5 PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα) A019D5 PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα) A019D5 FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody A019D5 FC
Spark YG™ 581 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα) A019D5 FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα) A019D5 PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody A019D5 FC
APC/Fire™ 750 anti-human CD127 A019D5 FC
PE anti-human CD127 A019D5 FC
PerCP/Cyanine5.5 anti-human CD127 A019D5 FC
PE/Cyanine7 anti-human CD127 A019D5 FC
Spark Red™ 718 anti-human CD127 (IL-7Rα) A019D5 FC
Go To Top Version: 1    Revision Date: 10/22/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD127 (IL-7Rα)

  • PE anti-human CD127 (IL-7Rα)

  • Pacific Blue™ anti-human CD127 (IL-7Rα)

  • Brilliant Violet 421™ anti-human CD127 (IL-7Rα)

  • FITC anti-human CD127 (IL-7Rα)

  • Alexa Fluor® 488 anti-human CD127 (IL-7Rα)

  • APC anti-human CD127 (IL-7Rα)

  • Alexa Fluor® 647 anti-human CD127 (IL-7Rα)

  • PE/Cyanine7 anti-human CD127 (IL-7Rα)

  • PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)

  • Brilliant Violet 570™ anti-human CD127 (IL-7Rα)

  • PE/Cyanine5 anti-human CD127 (IL-7Rα)

  • Brilliant Violet 650™ anti-human CD127 (IL-7Rα)

  • Brilliant Violet 711™ anti-human CD127 (IL-7Rα)

  • Brilliant Violet 785™ anti-human CD127 (IL-7Rα)

  • Brilliant Violet 510™ anti-human CD127 (IL-7Rα)

  • Brilliant Violet 605™ anti-human CD127 (IL-7Rα)

  • PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)

  • Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)

  • Alexa Fluor® 700 anti-human CD127 (IL-7Rα)

  • Biotin anti-human CD127 (IL-7Rα)

  • APC/Cyanine7 anti-human CD127 (IL-7Rα)

  • APC/Fire™ 750 anti-human CD127 (IL-7Rα)

  • TotalSeq™-A0390 anti-human CD127 (IL-7Rα)

  • TotalSeq™-B0390 anti-human CD127 (IL-7Rα)

  • TotalSeq™-C0390 anti-human CD127 (IL-7Rα)

  • KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)

  • Spark NIR™ 685 anti-human CD127 (IL-7Rα)

  • PE/Fire™ 640 anti-human CD127 (IL-7Rα)

  • PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody

  • Spark YG™ 581 anti-human CD127 (IL-7Rα)

  • Brilliant Violet 750™ anti-human CD127 (IL-7Rα)

  • TotalSeq™-D0390 anti-human CD127 (IL-7Rα)

  • APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody

  • APC/Fire™ 750 anti-human CD127

  • PE anti-human CD127

  • PerCP/Cyanine5.5 anti-human CD127

  • PE/Cyanine7 anti-human CD127

  • Spark Red™ 718 anti-human CD127 (IL-7Rα)

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