Alexa Fluor® 647 anti-mouse TCR Vβ2 Antibody

Pricing & Availability
Clone
B20.6 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vβ 2 [vb2]
Isotype
Rat IgG2a, κ
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Product Citations
publications
B20dot6_Alx647_070808.jpg
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and B20.6 Alexa Fluor™ 647
  • B20dot6_Alx647_070808.jpg
    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and B20.6 Alexa Fluor™ 647
See Alexa Fluor® 647 spectral data
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127910 100 µg 156,00€
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Description

The B20.6 antibody recognizes V beta 2 TCR of all strains tested. V beta 2 bearing T lymphocytes are clonally eliminated in MHC class II I-E expressing mice neonatally infected with MMTV virus through milk feeding.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone CW3/1.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References
  1. Necker A, et al. 1991. Eur J. Immunol. 21:3035
  2. Hodes RJ, et al. 1993. J Immunol 150:1422
Product Citations
  1. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
RRID
AB_1089254 (BioLegend Cat. No. 127910)

Antigen Details

Structure
TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution
On a subset of T cells
Function
Recognize MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with V alpha and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Immunology, Adaptive Immunity
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Necker A, et al. 1991. Eur. J. Immunol. 21:3035

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR V beta 2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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