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Ultra-LEAF™ Purified anti-mouse CD80 Antibody

Pricing & Availability
Clone
W17200C (See other available formats)
Regulatory Status
RUO
Other Names
B-Lymphocyte activation antigen B7.1, CD80, Ly53, TSA1, CD28 ligand (CD28L), MIC17
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17200C_ULEAF_CD80_Antibody_011922
Biotinylated recombinant mouse CTLA-4-Fc Chimera (Cat. No. 779106) (black circles) binds to immobilized recombinant mouse B7.1 (CD80)-Fc Chimera (Cat. No. 555406). Ultra-LEAF™ purified anti-mouse CD80 (clone W17200C) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.04 µg/mL biotinylated recombinant mouse CTLA-4-Fc Chimera to 1 µg/mL immobilized recombinant mouse B7.1 (CD80)-Fc Chimera. ND50 = 0.15 - 0.6 µg/mL
  • W17200C_ULEAF_CD80_Antibody_011922
    Biotinylated recombinant mouse CTLA-4-Fc Chimera (Cat. No. 779106) (black circles) binds to immobilized recombinant mouse B7.1 (CD80)-Fc Chimera (Cat. No. 555406). Ultra-LEAF™ purified anti-mouse CD80 (clone W17200C) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.04 µg/mL biotinylated recombinant mouse CTLA-4-Fc Chimera to 1 µg/mL immobilized recombinant mouse B7.1 (CD80)-Fc Chimera. ND50 = 0.15 - 0.6 µg/mL
  • W17200C_ULEAF_CD80_Antibody_2_011922
    C57BL/6 mouse splenocytes were stimulated with LPS for 3 days. Cells were stained with purified anti-mouse CD80 (clone W17200C, filled histogram) or Rat IgG2a, κ isotype control (open histogram), followed by goat anti-Rat IgG FITC
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600053 100 µg 172€
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600054 1 mg 572€
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Description

The B7 family of proteins are structurally related, cell membrane-associated molecules that regulate immune responses by delivering costimulatory or coinhibitory signals via their ligands. So far, eight family members have been identified, including B7.1 (CD80), B7.2, B7-H1, B7-H2, B7-H4, B7-H6, PD-L1, and PD-L2. These proteins are mainly expressed by activated antigen presenting cells (APCs) and when paired with either CD28 or CTLA-4 on a T lymphocyte, they induce an increase or decrease in the downstream signaling activity of the MHC-TCR interaction between APCs and T cells. B7.1 has been shown to bind both CTLA-4 and CD28. However, it has a 20-fold higher affinity to CTLA-4 than to CD28. Despite their structural similarities, the individual contribution of B7.1 and B7.2 to the development of pathogenic T cells in autoimmune diseases and protective T cells in infectious diseases is markedly distinct. Besides regulating T cell immune responses, B7 also plays a role in anti-tumor immunity. This is confirmed by demostrating that cytotoxic T cells were able to eradicate tumor cells transfected with B7.1 and B7.2. After activation, follicular lymphoma was shown to upregulate B7.1 and other costimulatory and adhesion molecules, therefore increasing APC activity and amplifying primed T cell responses. Homozygous null B7.1/B7.2 mice fail to generate antigen specific IgG1 and IgG2a responses, suggesting that B7 proteins are important in Ig immune responses. Mouse and human B7.1 share 44% amino acid identity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse B7.1 (CD80)-Fc Chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.04 µg/mL biotinylated recombinant mouse CTLA-4-Fc Chimera (Cat. No. 779106) to 1 µg/mL immobilized mouse B7.1 (CD80)-Fc Chimera (Cat. No. 555406). ND50 = 0.15 – 0.6 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2910468 (BioLegend Cat. No. 600053)
AB_2910468 (BioLegend Cat. No. 600054)

Antigen Details

Structure
Homodimer
Distribution

Activated B cells, monocytes, macrophages, dendritic cells

Function
Plays a role in T cell activation; CD80 and CD86 play an important role in T cell priming
Interaction
T cells
Ligand/Receptor
CD28, CTLA-4
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Freeman GJ, et al. 1993. Science. 262:909-11. 
  2. Azuma M, et al. 1993. Nature. 366:76-9. 
  3. Gimmi CD, et al. 1991. Proc Natl Acad Sci USA. 88:6575-9. 
  4. Linsley PS, et al. 1991. J Exp Med. 173:721-30. 
  5. Greaves P and Gribben JG. 2013. Blood. 121: 734-44. 
  6. Czuczman MS, et al. 2005. J Clin Oncol. 23:4390-8.
Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 05/15/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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