- CXCR3-173 (See other available formats)
- Regulatory Status
- Other Names
- CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- Mouse N-terminus of CXCR3
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
(PubMed link indicates BioLegend citation)
- Krug A, et al. 2002. J. Immunol. 169:6079.
- McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
- Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
- Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
- Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
- Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
- Uppaluri R, et al. 2008. Transplantation 86:137.
- Product Citations
AB_2832455 (BioLegend Cat. No. 126547)
AB_2572000 (BioLegend Cat. No. 126538)
AB_2832456 (BioLegend Cat. No. 126548)
AB_11147756 (BioLegend Cat. No. 126526)
AB_2571999 (BioLegend Cat. No. 126537)
T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
- Essential in T cell recruitment to sites of inflammation
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Gene ID
- 12766 View all products for this Gene ID
- View information about CD183 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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