Purified anti-VCP Antibody

Pricing & Availability
Clone
A18143C (See other available formats)
Regulatory Status
RUO
Other Names
Valosin-Containing Protein, CDC48
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18143C_PURE_VCP_Antibody_1_021920
Western blot of purified anti-VCP antibody (clone A18143C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18143C_PURE_VCP_Antibody_1_021920
    Western blot of purified anti-VCP antibody (clone A18143C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18143C_PURE_VCP_Antibody_2_021920
    IHC staining of purified anti-VCP antibody (clone A18143C) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18143C_PURE_VCP_Antibody_3_021920
    IHC staining of purified anti-VCP antibody (clone A18143C) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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870901 25 µg 72,00€
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870902 100 µg 188,00€
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Description

Valocin-containing protein (VCP) is a member of a family of proteins that include putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP associates with clathrin and heat-shock protein Hsc70 to form a complex that functions as a chaperon to unfold and transport proteins to different cellular compartments. Diseases associated with VCP include inclusion body myopathy with early-onset Paget's disease, and frontotemporal dementia 1 and amyotrophic lateral sclerosis 14, with or without frontotemporal dementia.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VCP Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2861105 (BioLegend Cat. No. 870901)
AB_2861106 (BioLegend Cat. No. 870902)

Antigen Details

Structure
VCP is an 806 amino acid protein with predicted molecular mass of ~89 kD and observed molecular mass at ~ 97 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol, endoplasmic reticulum, nucleus, extracellular, and lysosome

Function
Protein Trafficking, protein clearance
Interaction
Forms homohexamers and a ternary complex with STX5A and NSFLC. Interacts with NPLOC4, UFD1, and clathrin.
Cell Type
Astrocytes, Dopaminergic Neurons, Endothelial cells, Epithelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family
Endoplasmic Reticulum Markers, Neurotransmitter Receptor Trafficking/Endocytosis, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Lan B, et al. 2017. Curr Mol Med. 17(9):608.
  2. van den Boom J & Meyer H. 2018. Mol Cell. 69(2):182.
  3. Van Mossevelde S, et al. 2018. Nat Rev Neurol. 14(6):363.
Gene ID
7415 View all products for this Gene ID 269523 View all products for this Gene ID 116643 View all products for this Gene ID
UniProt
View information about VCP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/19/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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