Purified anti-mouse CD28 Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
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Product Citations
publications
37.51
C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
  • 37.51
    C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
Cat # Size Price Quantity Avail. Save
102101 50 µg 36,00€
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102102 500 µg 92,00€
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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, Costim, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Application References

(PubMed link indicates BioLegend citation)
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  2. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  3. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  4. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  5. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  6. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  7. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  8. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  9. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  10. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  11. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  12. He Y, et al. 2021. eLife. 10:00. PubMed
  13. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  14. Lu SX, et al. 2021. Cell. . PubMed
  15. Marangoni F, et al. 2021. Cell. . PubMed
  16. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  17. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  18. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  19. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  20. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  21. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  22. Racioppi L, et al. 2019. Nat Commun. 10:2450. PubMed
  23. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  24. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  25. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  26. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  27. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  28. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  29. Yamada T, et al. 2019. J Immunol. 202:1088. PubMed
  30. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  31. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  32. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  33. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  34. Schiering C, et al. 2017. Nature. 542:242. PubMed
  35. Majchrzak K, et al. 2017. JCI Insight. 2:e90547. PubMed
  36. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  37. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  38. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
  39. Oh H, et al. 2017. Immunity. 47:450. PubMed
  40. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  41. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  42. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  43. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  44. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  45. Thumkeo D, et al. 2020. Sci Adv. 6:eaay2432. PubMed
  46. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  47. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  48. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  49. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  50. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  51. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  52. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  53. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  54. Cross EW, et al. 2019. PLoS One. 14:e0211446. PubMed
  55. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  56. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  57. He N 2005. J Exp Med. 202:395. PubMed
  58. He N 2005. J Immunol. 174:6967. PubMed
  59. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  60. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  61. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  62. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  63. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  64. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
RRID
AB_312866 (BioLegend Cat. No. 102101)
AB_312867 (BioLegend Cat. No. 102102)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, CD4+, CD8+ peripheral T cells, NK cells

Function
Costimulates T and NK cells
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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