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Purified anti-MMP2 Antibody

Pricing & Availability
Clone
M6308B04 (See other available formats)
Regulatory Status
RUO
Other Names
Matrix metallopeptidase 2, Gelatinase A, MMP-2, GelA, Clg4a, CLG4, MONA, TBE-1, MMP-II
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
M6308B04_PURE_MMP2_Antibody_WB_092915
Recombinant human MMP2 treated with (lane 3; 50 ng) and without (lane 2; 50 ng) 1 mM of p-aminophenylmercuric acetate (APMA) and recombinant mouse MMP2 (lane 1; 50 ng) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP2 (clone M6308B04) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • M6308B04_PURE_MMP2_Antibody_WB_092915
    Recombinant human MMP2 treated with (lane 3; 50 ng) and without (lane 2; 50 ng) 1 mM of p-aminophenylmercuric acetate (APMA) and recombinant mouse MMP2 (lane 1; 50 ng) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP2 (clone M6308B04) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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680002 100 µg 172€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. No. 679902 is available as an alternative.
Description

MMP2, also named gelatinase A and MMP-2, is a member of matrix metalloproteinase family of proteins (MMPs). MMPs are structurally related enzymes that contain zinc that degrade the extracellular matrix and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling as well as in disease processes such as arthritis and metastasis. MMP2 consists of a prodomain, which is cleaved upon activation, a catalytic domain containing the zinc binding site, a fibronectin-like domain (that plays a role in the substrate targeting), and a carboxyl-terminal (hemopexin-like repeats) domain. The activation of MMP2 requires proteolytic processing starting first with a complex of membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP2 from the extracellular milieu to the cell surface. The second step of the process is MMP2 activating by active MT1-MMP and subsequent autocatalytic cleavage. Substrates of MMP2 include type IV collagen, aggrecan, link protein, decorin, fibronectin, and type X and XI collagens, which all are components of the articular cartilaginous matrix. Importantly, MMP2 secretion is elevated in several types of human cancers and its elevated expression has been associated with a poor prognosis. Mutations in the MMP2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. MMP2 deficient mice exhibit slightly delayed growth, reduced neovascularization, retarded tumor progression, an exaggerated asthma response to allergens, and impaired branching morphogenesis of the mammary gland.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MMP-2, amino acids (Ala30-Cys660) (Accession# NP_004521.1) with a C-terminal His tag and a linker sequence. It is expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Swieboda D, et al. 2020. Front Immunol. 1.697916667. PubMed
RRID
AB_2566323 (BioLegend Cat. No. 680002)

Antigen Details

Structure
Monomer. Human MMP2 contains 660 amino acids with a predicted molecular weight of 73.9 kD. Upon activation by p-aminophenylmercuric acetate (APMA), it has a predicted molecular weight of 62 kD.
Distribution

MMP2 is expressed by fibroblasts, endothelial cells, alveolar epithelial cells, and macrophages.

Function
MMP2 is a metalloproteinase that specifically cleaves type IV collagen, which is the major structural component of basement membranes. MMP2 is thought to contribute to the invasion and the metastasis of various human malignancies.
Interaction
Fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans, decorin, osteopontin, and IBP-3.
Ligand/Receptor
TIMPs, CLL7, and thrombospondin 1.
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts, Macrophages
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Nagase H and Woessner JF. 1999. J. Biol. Chem. 274:21491.
2. Martignetti JA, et al. 2001. Nat. Genet. 28:261.
3. Itoh T, et al. 1997. J. Biol. Chem. 272:22389.
4. Passlick B, et al. 2000. Clin. Cancer Res. 6:3944.
5. Qian Q, et al. 2010. Cancer Invest. 28:661.
6. Hoikkala S, et al. 2006. Cancer Lett. 236:125.
7. Yamamura T, et al. 2002. Cancer 35:249.

Gene ID
4313 View all products for this Gene ID
UniProt
View information about MMP-2 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 09/29/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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