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Purified anti-human TSLP Antibody

Pricing & Availability
Clone
13H8.3 (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal derived lymphopoietin
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
512102 500 µg 254€
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Description

Thymic Stromal Lymphopoietin (TSLP) is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. Recent studies revealed that expression of TSLP has a direct link to the pathogenesis of allergic inflammation such as atopic dermatitis and asthma via activating dendritic cells and mast cells which trigger inflammatory Th2 response characterized by high TNF-alpha and little IL-10 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA
Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TSLP recombinant protein ranging from 200 to 3.1 ng/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
RRID
AB_940339 (BioLegend Cat. No. 512102)

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
1. Liu YJ. 2007. J Allergy Clin Immunol. 120:238. Nagata Y. 2007 Int Arch Allergy Immunol. 144:305.
2. Jiang Q, et al. 2007 BMC Immunol. 8:11.
3. Kato A. et al. 2007. J Immunol. 179:1080.
4. Rochman I, et al. 2007 J Immunol. 178(11):6720.
5. Reche PA, et al. 2001. J Immunol. 167:336.
6. Pandey A, et al. 2000. Nature Immunol. 1:59.
7. Park LS, et al. 2000. J Exp. Med. 192:659.
Gene ID
85480 View all products for this Gene ID
UniProt
View information about TSLP on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-human TSLP

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