Purified anti-human IL-10 (Maxpar® Ready) Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Rhesus, Cynomolgus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS - expressed, recombinant human IL-10

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

ELISA Capture^1-5 or ELISPOT Capture⁶: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization^1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include: immunohistochemical staining¹².
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
4. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
6. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
11. Mason GM, et al. 2012. PNAS. PubMed
12. 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)

Product Citations

1. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed

RRID

AB_2563778 (BioLegend Cat. No. 501423)

Antigen Details

Structure

Acid-labile cytokine, dimer, 35-40 kD (Mammalian)

Bioactivity

Inhibit IFN-γ, TNF-β, IL-2 production by T_H1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate T_H2 cell response; mast cell proliferation in

Cell Sources

Activated CD8⁺ and CD4⁺ T cells, activated monocytes, mast cells, Ly-1 B (mouse)

Cell Targets

T cells, B cells, mast cells, macrophages

Receptors

IL-10R (CDw210)

Cell Type

Tregs

Biology Area

Cell Biology, Immunology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation

Production inhibited by IL-4, IL-10

Gene ID

3586 View all products for this Gene ID

UniProt

View information about IL-10 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

Certificate of Analysis