Purified anti-ERp57 Antibody

Pricing & Availability
Clone
W19080B (See other available formats)
Regulatory Status
RUO
Other Names
Protein Disulfide Isomerase Family A Member 3, PDIA3, Endoplasmic Reticulum Resident Protein 57, GRP58, ER Protein 57, GRP57, Epididymis Secretory Protein Li 269, Epididymis Scretory Sperm Binding Protein Li 93n
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19080B_PURE_ERp57_Antibody_1_061020
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERp57 antibody (clone W19080B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19080B_PURE_ERp57_Antibody_1_061020
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERp57 antibody (clone W19080B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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937301 25 µg 68,00€
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937302 100 µg 172,00€
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Description

ERp57 is a member of the protein disulfide family of endoplasmic reticulum localized oxidoreductases and plays an important role in disulfide bond formation and protein folding. Along with calnexin and calreticulin, it is essential for the synthesis and quality control of nascent glycoproteins in the secretory pathway. It is also a core component of the peptide-loading complex that is required for MHC class I presentation. ERp57 is upregulated in multiple neurodegenerative diseases and cancers, and targeted disruption of ERp57 sensitizes cancer cells to chemotherapeutic agents and ionizing radiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human ERp57 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed IP testing.

RRID
AB_2861126 (BioLegend Cat. No. 937301)
AB_2861127 (BioLegend Cat. No. 937302)

Antigen Details

Structure
ERp57 is a 505 amino acid protein with a predicted molecular weight of 57 kD.
Distribution

Endoplasmic reticulum/Ubiquitously expressed

Function
Molecular chaperone
Biology Area
Cell Biology, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Endoplasmic Reticulum Markers, Enzymes and Regulators
Antigen References
  1. Oliver JD, et al. 1997. Science. 275:86-8.
  2. Kienast A, et al. 2007. Nat Immunol. 8:864-72.
  3. Hussman M, et al. 2015. Oncotarget. 6:39247-61.
  4. Blees A, et al. 2017. Nature. 551:525-528.
Gene ID
2923 View all products for this Gene ID
UniProt
View information about ERp57 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/10/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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