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- Clone
- 4D6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease (autosomal dominant, Lewy body) 4
- Previously
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Signet Catalog# 9720-02
Signet Catalog# 9720-05
Signet Catalog# 9720-10
Covance Catalog# SIG-39720
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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IHC staining of purified anti-α-Synuclein antibody (clone 4D6) on formalin-fixed paraffin-embedded Parkinson's disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Western blot of purified anti-α-Synuclein antibody (clone 4D6) (image left) and isotype-matched IgG1 control (image right). Lane 1, 6: Molecular weight marker; Lane 2, 7: 20 µg of human brain lysate; Lane 3, 8: 20 µg of Parkinson's disease human brain lysate; Lane 4, 9: 20 µg of mouse brain lysate; Lane 5, 10: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of the clone 4D6 or mouse IgG1 overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
α-synuclein, Alpha-synuclein, is expressed principally in the central nervous system (brain) but is also expressed in low concentrations in a variety of tissues except liver. It is predominantly expressed in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum of the CNS. It is primarily a neuronal protein, but can also be found in the neuroglial cells. It is concentrated in presynaptic nerve terminals of neurons, as well as having reported nuclear and mitochondrial localization. α-synuclein interacts with plasma membrane phospholipids. α-synuclein in solution is considered to be an intrinsically disordered protein and thus lacks a stable secondary or tertiary structure. However, recent data suggests the presence of partial alpha helical as well as beta sheet structures as well as mostly structured tetrameric states in solution, the equilibrium of which may be altered by binding partners. The human α-synuclein protein is made of 140 amino acids, encoded by the SNCA gene. The primary structure is divided in three distinct domains: (1-60) - An amphipathic N-terminal region dominated by four 11-residue repeats including the consensus sequence KTKEGV. This sequence has a structural alpha helix propensity similar to apolipoproteins-binding domains. (61-95)- a central hydrophobic region which includes the non-amyloid-β component (NAC) region, involved in protein aggregation. (96-140)- a highly acidic and proline-rich region. At least three isoforms of synuclein are produced through alternative splicing. The most common form of the protein, is the full 140 amino acid-long transcript. Other isoforms are alpha-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein may be involved in the regulation of dopamine release and transport and also may function to induce fibrillization of microtubule-associated protein tau. α-synuclein functions as a molecular chaperone in the formation of SNARE complexes. In particular, it can bind to phospholipids of the plasma membrane and to synaptobrevin-2 via its C-terminus domain to influence synaptic activity. α-synuclein is essential for normal development of the cognitive functions and that it significantly interacts with tubulin. It also reduces neuronal responsiveness to various apoptotic stimuli, leading to decreased caspase-3 activation. α-Synuclein fibrils are major substituent of the intracellular Lewy bodies seen in Parkinson's disease.
Alpha-synuclein is a 140 amino acid protein that is implicated in Parkinson’s disease, Alzheimer’s disease, Lewy body dementia, and multiple system atrophy.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using a purified, E. Coli produced human α-synuclein.
- Formulation
- Phosphate-buffered solution; no preservatives or carrier proteins.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
BioLegend’s 4D6 reacts with human, mouse, and rat a-synuclein. It does not react with ß-synuclein or ?-synuclein.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2564985 (BioLegend Cat. No. 834303)
AB_2734600 (BioLegend Cat. No. 834304)
AB_2564986 (BioLegend Cat. No. 834301)
AB_2564987 (BioLegend Cat. No. 834302)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein
- Gene ID
- 6622 View all products for this Gene ID
- UniProt
- View information about alpha-Synuclein on UniProt.org
Related FAQs
Other Formats
View All α-Synuclein Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-α-Synuclein | 4D6 | IHC-P,WB |
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