PerCP/Cyanine5.5 anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_PerCPCy5dot5_011108
C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5
  • 104_PerCPCy5dot5_011108
    C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
109827 25 µg 56€
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109828 100 µg 180€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  2. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  3. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  4. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  5. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  6. Yap JY, et al. 2018. Immunology. 154:522. PubMed
  7. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  8. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  9. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  10. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  11. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  12. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  13. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  14. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  15. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  16. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  17. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  18. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  19. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  20. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  21. Forero A, et al. 2020. Immunity. 51(3):451-464. PubMed
  22. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  23. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  24. MacKinnon A, et al. 2011. Am J Respir Crit Care Med. 185:537. PubMed
  25. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  26. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  27. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  28. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  29. Kan X, et al. 2015. Cardiovasc Res. 10.1093/cvr/cvv255. PubMed
  30. Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed
  31. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  32. Rodriguez-Ruiz M, et al. 2016. Cancer Res . 76: 5994 - 6005. PubMed
  33. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  34. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  35. Emal D, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016030255. PubMed
  36. Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed
RRID
AB_893352 (BioLegend Cat. No. 109827)
AB_893350 (BioLegend Cat. No. 109828)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 09/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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