PerCP/Cyanine5.5 anti-human CD38 Antibody

Pricing & Availability
Clone
HIT2 (See other available formats)
Regulatory Status
RUO
Workshop
III 155
Other Names
T10, ADP-ribosyl cyclase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HIT2_PerCPCyanine55_CD38_Antibody_120418
Human peripheral blood lymphocytes were stained with CD38 (clone HIT2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • HIT2_PerCPCyanine55_CD38_Antibody_120418
    Human peripheral blood lymphocytes were stained with CD38 (clone HIT2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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303521 25 tests 132€
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303522 100 tests 287€
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Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Horse, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and spatial biology (IBEX)10,11.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373.
  3. Esser M, et al. 2001. J. Virol. 75:6173.
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044.
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107.
  6. van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC)
  7. Bende RJ, et al. 2003. Am. J. Pathol. 162:105.
  8. Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang Z, et al. 2018. Nat Commun. 9:824. PubMed
  2. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  3. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  4. Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed
  5. Mesa-Nuñez C, et al. 2020. Stem Cell Res Ther. 11:164. PubMed
  6. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
  7. Planchais C, et al. 2018. J Immunol. 200:3519. PubMed
  8. Han L, et al. 2012. PLoS One. 4:e7989. PubMed
  9. Simmons R, et al. 2013. J Virol. 87:3087. PubMed
  10. Bluestone J, et al. 2015. Sci Transl Med. 7: 315ra189. PubMed
  11. Kerkman PF, Kempers AC 2016. Ann Rheum Dis. 75(12):2201-2207. PubMed
  12. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  13. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
RRID
AB_893316 (BioLegend Cat. No. 303521)
AB_893314 (BioLegend Cat. No. 303522)

Antigen Details

Structure
ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution

T cells, B cells, NK, myeloid, plasma, and dendritic cells

Function
Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor
CD31, hyaluronic acid
Cell Type
B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469.

Gene ID
952 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD38 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD38 HIT2 FC
FITC anti-human CD38 HIT2 FC
PE anti-human CD38 HIT2 FC
PE/Cyanine5 anti-human CD38 HIT2 FC
Purified anti-human CD38 HIT2 FC, CyTOF®, IHC-F
Alexa Fluor® 488 anti-human CD38 HIT2 FC
Alexa Fluor® 647 anti-human CD38 HIT2 FC
PE/Cyanine7 anti-human CD38 HIT2 FC
Biotin anti-human CD38 HIT2 FC
PerCP anti-human CD38 HIT2 FC
PerCP/Cyanine5.5 anti-human CD38 HIT2 FC
Alexa Fluor® 700 anti-human CD38 HIT2 FC, SB
Brilliant Violet 421™ anti-human CD38 HIT2 FC
Brilliant Violet 711™ anti-human CD38 HIT2 FC
Brilliant Violet 785™ anti-human CD38 HIT2 FC
Brilliant Violet 605™ anti-human CD38 HIT2 FC
APC/Cyanine7 anti-human CD38 HIT2 FC
Purified anti-human CD38 (Maxpar® Ready) HIT2 FC, CyTOF®
PE/Dazzle™ 594 anti-human CD38 HIT2 FC
PE anti-human CD38 HIT2 FC
Brilliant Violet 510™ anti-human CD38 HIT2 FC
FITC anti-human CD38 HIT2 FC
PE/Dazzle™ 594 anti-human CD38 HIT2 FC
TotalSeq™-A0389 anti-human CD38 HIT2 PG
TotalSeq™-C0389 anti-human CD38 HIT2 PG
APC/Fire™ 750 anti-human CD38 HIT2 FC
TotalSeq™-B0389 anti-human CD38 HIT2 PG
APC/Fire™ 810 anti-human CD38 HIT2 FC
Spark NIR™ 685 anti-human CD38 Antibody HIT2 FC
TotalSeq™-D0389 anti-human CD38 HIT2 PG
PerCP/Cyanine5.5 anti-human CD38 HIT2 FC
APC anti-human CD38 HIT2 FC
APC/Fire™ 750 anti-human CD38 HIT2 FC
PE/Cyanine7 anti-human CD38 HIT2 FC
GMP PE anti-human CD38 HIT2 FC
GMP FITC anti-human CD38 HIT2 FC
Pacific Blue™ anti-human CD38 HIT2 FC
Go To Top Version: 5    Revision Date: 12/17/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD38

  • FITC anti-human CD38

  • PE anti-human CD38

  • PE/Cyanine5 anti-human CD38

  • Purified anti-human CD38

  • Alexa Fluor® 488 anti-human CD38

  • Alexa Fluor® 647 anti-human CD38

  • PE/Cyanine7 anti-human CD38

  • Biotin anti-human CD38

  • PerCP anti-human CD38

  • PerCP/Cyanine5.5 anti-human CD38

  • Alexa Fluor® 700 anti-human CD38

  • Brilliant Violet 421™ anti-human CD38

  • Brilliant Violet 711™ anti-human CD38

  • Brilliant Violet 785™ anti-human CD38

  • Brilliant Violet 605™ anti-human CD38

  • APC/Cyanine7 anti-human CD38

  • Purified anti-human CD38 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD38

  • PE anti-human CD38

  • Brilliant Violet 510™ anti-human CD38

  • FITC anti-human CD38

  • PE/Dazzle™ 594 anti-human CD38

  • TotalSeq™-A0389 anti-human CD38

  • TotalSeq™-C0389 anti-human CD38

  • APC/Fire™ 750 anti-human CD38

  • TotalSeq™-B0389 anti-human CD38

  • APC/Fire™ 810 anti-human CD38

  • Spark NIR™ 685 anti-human CD38 Antibody

  • TotalSeq™-D0389 anti-human CD38

  • PerCP/Cyanine5.5 anti-human CD38

  • APC anti-human CD38

  • APC/Fire™ 750 anti-human CD38

  • PE/Cyanine7 anti-human CD38

  • GMP PE anti-human CD38

  • GMP FITC anti-human CD38

  • Pacific Blue™ anti-human CD38

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