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PerCP/Cyanine5.5 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UCHT1_PerCPCyanine55_CD3_Antibody_110518
Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • UCHT1_PerCPCyanine55_CD3_Antibody_110518
    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
300429 25 tests 141€
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300430 100 tests 306€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Poonia B, Pauza C 2014. PLoS One. 9:88884. PubMed
  2. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  3. Xu L, et al. 2018. Mol Oncol. 12:269. PubMed
  4. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  5. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  6. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  7. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  8. Yeung Y, et al. 2016. Nat Commun. 7:13376. PubMed
  9. Armour K, et al. 2014. PLoS One. 9:109463. PubMed
  10. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  11. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  12. Rajakumar SA, et al. 2021. Cell Rep Med. 2:100470. PubMed
  13. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  14. Mbiribindi B, et al. 2020. Sci Rep. 10:19973. PubMed
  15. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  16. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  17. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  18. Zhang M, et al. 2020. J Cell Mol Med. . PubMed
  19. Jilkova Z, et al. 2017. Mediators Inflamm. 10.1155/2017/3214917. PubMed
  20. van Heerden D, et al. 2021. Int J Mol Sci. 22:. PubMed
  21. Turner AW, et al. 2020. ACS Infect Dis. 6:1719. PubMed
  22. Shen M, et al. 2018. Int J Oncol. 53:317. PubMed
  23. Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
  24. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
  25. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  26. Aydin AM, et al. 2021. Int Immunopharmacol. 94:107481. PubMed
  27. Xu S, et al. 2021. Immunity. . PubMed
  28. Sumitomo S, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.001. PubMed
  29. Vendrame E, et al. 2020. AIDS. 34:801. PubMed
  30. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  31. Pritchett J, et al. 2016. J Infect Dis. 214(12):1911-1915. PubMed
  32. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  33. Nakachi S, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1309-x. PubMed
  34. Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed
  35. Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
  36. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  37. Landgraf KE, et al. 2020. Commun Biol. 0.330555556. PubMed
  38. Gross C, et al. 2016. Proc Natl Acad Sci U S A. 113: 2973 - 2982. PubMed
  39. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  40. Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
  41. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  42. Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed
  43. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  44. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  45. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  46. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
  47. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  48. Poznanski SM, et al. 2021. Cell Metabolism. 33(6):1205-1220.e5. PubMed
  49. Ribot J, et al. 2014. J Immunol. 192:2237. PubMed
  50. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  51. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
RRID
AB_893301 (BioLegend Cat. No. 300429)
AB_893299 (BioLegend Cat. No. 300430)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 2    Revision Date: 11/05/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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