PE/Cyanine7 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
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Product Citations
publications
M1slash70_PECy7_042706
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram) (gated on total cells).
  • M1slash70_PECy7_042706
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram) (gated on total cells).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
101215 25 µg 64,00€
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101216 100 µg 156,00€
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed
  2. Gao C, et al. 2017. Mol Neurobiol.. 10.1007/s12035-016-0318-0. PubMed
  3. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  4. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  5. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  6. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  7. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  8. McGill BE et al. 2018. The Journal of Neuroscience. 38(1):200-219 . PubMed
  9. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  10. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  11. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  12. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  13. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  14. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  15. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  16. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  17. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  18. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  19. Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed
  20. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  21. Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed
  22. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  23. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  24. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  25. Saggu G, et al. 2018. Nat Commun. 9:5058. PubMed
  26. Akhmetzyanova ER, et al. 2019. Front Cell Neurosci. 12:507. PubMed
  27. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  28. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  29. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  30. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  31. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  32. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  33. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  34. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  35. Saul S, et al. 2019. J Leukoc Biol. N/A. PubMed
  36. Lalor R, et al. 2019. Nutrients. 11. PubMed
  37. Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
  38. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  39. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  40. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  41. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  42. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  43. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  44. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  45. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  46. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  47. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  48. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  49. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  50. Gibson EM, et al. 2019. Cell. 176:43:00. PubMed
  51. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  52. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  53. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  54. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  55. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  56. Secondini C, et al. 2017. Oncoimmunology. 6:e1316437. PubMed
  57. Vasilijevic J, et al. 2017. PLoS Pathog. 13:e1006650. PubMed
  58. Kobayashi Y, et al. 2018. PLoS One. 13:e0195008. PubMed
  59. Okunuki Y, et al. 2018. Proc Natl Acad Sci U S A. 115:E6264. PubMed
  60. Rosen DA, et al. 2019. Sci Transl Med. 11:eaau5266. PubMed
  61. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  62. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  63. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  64. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  65. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  66. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  67. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  68. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  69. Dokoshi T, et al. 2020. Cell Rep. 30:61. PubMed
  70. Wilson DH, et al. 2020. Nat Commun. 0.767361111. PubMed
  71. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  72. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  73. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  74. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  75. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  76. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  77. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  78. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  79. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  80. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  81. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  82. Endoh M, et al. 2020. Cell Reports. 30(6):1823-1834. PubMed
  83. Süß P, et al. 2020. Cell Reports. 30(12):4082-4095. PubMed
  84. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  85. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  86. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  87. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  88. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  89. Alfaro M, et al. 2008. Proc Natl Acad Sci U S A. 105:18366. PubMed
  90. Meeks K, et al. 2009. J Immunol. 183:8026. PubMed
  91. Beauchamp N, et al. 2010. J Virol. 84:10191. PubMed
  92. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
  93. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  94. Gharib S, et al. 2014. J Leukoc Biol. 95:9. PubMed
  95. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  96. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  97. Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed
  98. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  99. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  100. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  101. Tebartz C, et al. 2015. J Immunol. 194:1100. PubMed
  102. Boulay A, et al. 2015. J Neurosci. 35:4427. PubMed
  103. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  104. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  105. Shen H, et al. 2015. Infect Immun . 83: 2694 - 2704. PubMed
  106. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  107. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  108. Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed
  109. Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed
  110. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  111. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  112. Arce-Cerezo A, et al. 2015. Sci Rep. 5: 1448. PubMed
  113. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  114. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  115. Groppa E, et al. 2015. EMBO Mol Med. 2015; 7: 1366 - 1384. PubMed
  116. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  117. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  118. Moro T, et al. 2016. PLoS One. 11: 0146592. PubMed
  119. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  120. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  121. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  122. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
  123. Kirschbaum K, et al. 2016. Proc Natl Acad Sci U S A. 113: 13227 - 13232. PubMed
  124. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  125. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  126. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  127. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  128. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  129. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  130. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  131. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  132. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  133. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  134. Gao P, et al. 2020. Front Immunol. 1.636111111. PubMed
  135. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  136. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  137. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  138. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  139. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  140. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  141. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  142. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  143. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  144. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  145. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  146. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  147. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  148. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  149. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  150. Liu K, et al. 2021. Cell Metabolism. 33(3):666-675.e4. PubMed
  151. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  152. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  153. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  154. Young K, et al. 2021. Cell Stem Cell. . PubMed
  155. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  156. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
RRID
AB_312798 (BioLegend Cat. No. 101215)
AB_312799 (BioLegend Cat. No. 101216)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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