PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse bone marrow mast cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of acetone fixed frozen sections², and spatial biology (IBEX)^5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

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RRID

AB_313222 (BioLegend Cat. No. 105813)
AB_313223 (BioLegend Cat. No. 105814)

Antigen Details

Structure

Ig superfamily, 145 kD

Distribution

Hematopoietic stem cells, AML, mast cells

Function

Growth factor receptor, tyrosine kinase

Ligand/Receptor

Stem Cell Factor (SCF)

Cell Type

Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells

Biology Area

Immunology, Stem Cells

Molecular Family

CD Molecules

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID

16590 View all products for this Gene ID

UniProt

View information about CD117 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis