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PE anti-mouse CD105 Antibody

Pricing & Availability
Clone
MJ7/18 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Rat IgG2a, κ
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Product Citations
publications
MJ7slash18_PE_070609.jpg
BEND.3, mouse endothelial cells stained with MJ7/18 PE
  • MJ7slash18_PE_070609.jpg
    BEND.3, mouse endothelial cells stained with MJ7/18 PE
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120407 25 µg 74€
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120408 100 µg 176€
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Description

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Inflamed mouse skin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Ge AZ and Butcher EC. 1994. Gene 138:201.
  2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
  3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
  4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  3. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  4. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  5. Huang Z, et al. 2020. Mol Med Rep. 1.979166667. PubMed
  6. Mittal S, et al. 2016. Stem Cell Reports. 7:583-590. PubMed
  7. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  8. Zhang W, et al. 2021. Cell Prolif. 54:e13058. PubMed
  9. Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed
  10. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  11. Zhao X, et al. 2022. Biomed Opt Express. 13:791. PubMed
  12. Babazadeh S, et al. 2021. Cell Mol Biol Lett. 26:30. PubMed
  13. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  14. Nakagawa MM, et al. 2018. Cell Rep. 25:2094. PubMed
  15. Franklin R, et al. 2022. Nat Commun. 13:2350. PubMed
  16. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
RRID
AB_1027701 (BioLegend Cat. No. 120407)
AB_1027699 (BioLegend Cat. No. 120408)

Antigen Details

Structure
Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution

Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast

Function
Suppresses TGF-β signaling, angiogenesis
Ligand/Receptor
TGF-β1, TGF-β3
Cell Type
Endothelial cells, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.

Gene ID
13805 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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