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PE anti-human TCR Vβ23 Antibody

Pricing & Availability
Clone
αHUT7 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vbeta 23
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
aHUT7_PE_1_062911
Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vβ23 (clone αHUT7) PE (top), or mouse IgG1, κ PE isotype control (bottom).
  • aHUT7_PE_1_062911
    Human peripheral blood lymphocytes were stained with CD3 FITC and TCR Vβ23 (clone αHUT7) PE (top), or mouse IgG1, κ PE isotype control (bottom).
  • aHUT7_PE_2_062911
See PE spectral data
Cat # Size Price Quantity Check Availability Save
349406 100 tests 268€
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Description

Vβ23 T cell receptor (TCR Vβ23) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of αβ+ T cells. TCR α/β recognizes peptides presented by the MHC molecules and initiates T cell mediated immune responses.

Vβ23+ T cells are stimulated by the staphylococcal enterotoxin A (SEA) and by the pyrogenic toxin superantigens (PTSAgs) SpeK, SpeL, and SpeM from streptococcus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T-cell leukemia line HUT78
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: stimulation of HUT78 T cells to produce IL-21.

Application References

(PubMed link indicates BioLegend citation)
  1. Romagne F, et al. 1995. J. Immunol. Methods 186:313. (Stim)
  2. Rebai N, et al. 1994. P. Natl. Acad. Sci. USA 91:1529. (FC)
  3. Pantaleo G, et al. 1997. P. Natl. Acad. Sci. USA 94:9848. (FC)
RRID
AB_10918433 (BioLegend Cat. No. 349406)

Antigen Details

Structure
Immunoglobulin superfamily, associate with TCR Vα chain and CD3 complex
Distribution

subset of TCR αβ+ T cells

Function
Recognition of peptides presented by MHC molecules, responsible for T cell mediated immune response
Ligand/Receptor
MHC-peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Guilherme L, et al. 2007. Curr. Protein Pept. Sc. 8:39.
2. Adelman MK. 2007. Crit. Rev. Immunol. 27:221.
3. Beres SB, et al. 2002. P. Natl. Acad. Sci. USA 99:10078.
4. Smoot LM, et al. 2002. Infect. Immunol. 70:7095.
5. Lavoie PM, et al. 2005. Int. Immunol. 17:931.

Gene ID
6957 View all products for this Gene ID
UniProt
View information about TCR Vbeta23 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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