Pacific Blue™ anti-mouse CD106 Antibody

Pricing & Availability
Clone
429 (MVCAM.A) (See other available formats)
Regulatory Status
RUO
Other Names
VCAM-1, INCAM-110
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_429_PB_100609
C57BL/6 bone marrow cells stained with 429 Pacific Blue™ and CD11b (M1/70) PE
  • A_429_PB_100609
    C57BL/6 bone marrow cells stained with 429 Pacific Blue™ and CD11b (M1/70) PE
  • B_429_PB_100609
    C57BL/6 bone marrow cells stained with rat IgG2a isotype control Pacific Blue™ and CD11b (M1/70) PE
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
105722 100 µg 200,00€
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Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α41 integrin) and LPAM-1 (α47 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse preadipose cell line PA6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, and immunoprecipitation1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
  2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
  4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
  6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
  7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
  9. Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
  10. Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
RRID
AB_2304290 (BioLegend Cat. No. 105722)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α41 integrin) and LPAM-1 (α47 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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