The LEGENDplex™ Human Inflammation Panel 2 Standard contains 13 human proteins, including TGF-b1, PAI-1, sTREM-1, PTX3, sCD40L, sCD25 (IL-2Ra), CXCL12 (SDF-1), sST2, sTNF-RI, sTNF-RII, sRAGE, CX3CL1 (Fractalkine), sCD130 (gp130). This standard cocktail is intended for use with the following in Mix and Match assays:
Human Inflammation Panel 2
740777 LEGENDplex™ Human Inflammation Panel 2 Detection Antibodies
740779 LEGENDplex™ Human Free Active TGF-b1 Capture Bead A4, 13X
740780 LEGENDplex™ Human PAI-1 Capture Bead A5, 13X
740781 LEGENDplex™ Human sTREM-1 Capture Bead A6, 13X
740782 LEGENDplex™ Human PTX3 Capture Bead A7, 13X
740783 LEGENDplex™ Human sCD40L Capture Bead A8, 13X
740784 LEGENDplex™ Human sCD25 (IL-2Ra) Capture Bead A10, 13X
740785 LEGENDplex™ Human CXCL12 (SDF-1) Capture Bead B2, 13X
740786 LEGENDplex™ Human sST2 Capture Bead B3, 13X
740787 LEGENDplex™ Human sTNF-RI Capture Bead B4, 13X
740788 LEGENDplex™ Human sTNF-RII Capture Bead B5, 13X
740789 LEGENDplex™ Human sRAGE Capture Bead B6, 13X
740790 LEGENDplex™ Human CX3CL1 (Fractalkine) Capture Bead B7, 13X
740791 LEGENDplex™ Human sCD130 (gp130) Capture Bead B9, 13X
740620 LEGENDplex™ Buffer Set H
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-Bottom Plate
- Biology Area
- Cell Biology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.