- Regulatory Status
- Other Names
- Cancer, Metastasis, Tumor, LEGENDplex, ICAM-1, CD54, ICAM-2, CD102, ICAM-3, CD50, VCAM-1, CD106, PECAM-1, CD31, ALCAM-1, CD166, EpCAM, CD326, NCAM, CD56, E-selectin, P-selectin, L-selectin, PSGL-1, CD44
- Ave. Rating
- Submit a Review
- Product Citations
The LEGENDplex™ Human Adhesion Molecule Panel Standard contains 13 recombinant human proteins: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), VCAM-1 (CD106), PECAM-1 (CD31), ALCAM-1 (CD166), EpCAM (CD326), NCAM (CD56), E-selectin, P-selectin, L-selectin, PSGL-1, and CD44. These standards are intended for use with the following in Mix and Match assays:
Human Adhesion Molecule Panel
740947 LEGENDplex™ Human Adhesion Molecule Panel Detection Antibodies
740949 LEGENDplex™ Human CD44 Capture Bead A4, 13X
740950 LEGENDplex™ Human ICAM-1 Capture Bead A5, 13X
740951 LEGENDplex™ Human ICAM-2 Capture Bead A6, 13X
740898 LEGENDplex™ Human PSGL-1 Capture Bead A7, 13X
740952 LEGENDplex™ Human NCAM Capture Bead A8, 13X
740953 LEGENDplex™ Human ALCAM Capture Bead A10, 13X
740954 LEGENDplex™ Human VCAM-1 Capture Bead B2, 13X
740955 LEGENDplex™ Human E-selectin Capture Bead B3, 13X
740956 LEGENDplex™ Human PECAM-1 Capture Bead B4, 13X
740957 LEGENDplex™ Human P-selectin Capture Bead B5, 13X
740958 LEGENDplex™ Human EpCAM Capture Bead B6, 13X
740959 LEGENDplex™ Human ICAM-3 Capture Bead B7, 13X
740960 LEGENDplex™ Human L-selectin Capture Bead B9, 13X
740539 LEGENDplex™ Buffer Set G (Set Up Beads, Buffers, Plate Sealers, SA-PE, and Lyophilized Standard Reconsitution Buffer)
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-Bottom Plate
- Biology Area
- Cell Adhesion, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.