- Regulatory Status
- Other Names
- Multiplex assay, Flex set, CBA, chemotactic cytokine, CXCL8 (IL-8), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL3 (MIP-1α), CXCL9 (MIG), CCL5 (RANTES), CCL20 (MIP-3α), CXCL1 (GROα), CXCL11 (I-TAC), CCL4 (MIP-1β), MCP-1
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- Product Citations
The LEGENDplex™ Human Proinflammatory Chemokine Panel 1 Standard contains 10 human proteins including IL-8, IP-10, Eotaxin, TARC, RANTES, MIP-1α, MIG, MIP-3α, GROα, I-TAC, MIP-1β, MCP-1, ENA-78. This Standard cocktail is intended for use with the following in Mix and Match assay reagents:
740988 LEGENDplex™ Human CXCL8 (IL-8) Capture Bead A4, 13X
740989 LEGENDplex™ Human CXCL10 (IP-10) Capture Bead A5, 13X
740990 LEGENDplex™ Human CCL11 (Eotaxin) Capture Bead A6, 13X
740991 LEGENDplex™ Human CCL17 (TARC) Capture Bead A7, 13X
740992 LEGENDplex™ Human CCL5 (RANTES) Capture Bead A10, 13X
740993 LEGENDplex™ Human CCL3 (MIP-1α) Capture Bead B2, 13X
740994 LEGENDplex™ Human CXCL9 (MIG) Capture Bead B3, 13X
740995 LEGENDplex™ Human CCL20 (MIP-3α) Capture Bead B5, 13X
740996 LEGENDplex™ Human CXCL1 (GROα) Capture Bead B6, 13X
740997 LEGENDplex™ Human CXCL11 (I-TAC) Capture Bead B7, 13X
740998 LEGENDplex™ Human CCL4 (MIP-1β) Capture Bead B9, 13X
740813 LEGENDplex™ Human MCP-1 Capture Bead A8, 13X
740084 LEGENDplex™ Human CXCL5 (ENA-78) Capture Bead B4, 13X
740987 LEGENDplex™ HU Proinflam. Chemokine Panel 1 Standard
740368 LEGENDplex™ Buffer Set A
740986 LEGENDplex™ Human Proinflammatory Chemokine Panel 1 Detection Antibodies
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-bottom Plate
- Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.