FITC anti-mouse Ly-6D Antibody

Pricing & Availability
49-H4 (See other available formats)
Regulatory Status
Other Names
Lymphocyte antigen 6D, Thymocyte B-cell antigen, ThB
Rat IgG2c, κ
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Product Citations
C57BL6 mouse splenocytes were stained with CD19 APC and Ly-6D (clone 49-H4) FITC (top) or rat IgG2c, κ APC isotype control (bottom).
  • 49-H4_FITC_1_030812
    C57BL6 mouse splenocytes were stained with CD19 APC and Ly-6D (clone 49-H4) FITC (top) or rat IgG2c, κ APC isotype control (bottom).
  • 49-H4_FITC_2_030812
See FITC spectral data
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138605 25 µg 60,00€
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138606 100 µg 176,00€
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Lymphocyte antigen 6D (Ly-6D), also known as thymocyte B-cell antigen (ThB), is a 16 kD GPI-linked cell surface glycoprotein. It belongs to the Ly6 gene family and contains one urokinase receptor (uPAR) /Ly6 domain. Ly-6D is involved in cell-cell adhesion and signal transduction. Ly-6D marks the earliest stage of B cell lineage commitment from the common lymphoid progenitors (CLP). It is expressed on B cells, immature thymocytes, and plasmacytoid dendritic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
BALB/c mouse plasmacytoma MOPC-104E
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Eckhardt LA and Herzenberg LA. 1980. Immunogenetics 11:275. (FC)
Product Citations
  1. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  2. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  3. Janela B, et al. 2019. Immunity. 50:1069. PubMed
AB_11203901 (BioLegend Cat. No. 138605)
AB_11203888 (BioLegend Cat. No. 138606)

Antigen Details

GPI-linked cell-surface glycoprotein, molecular weight 16 kD, belongs to the Ly6 gene family, contains one urokinase receptor (uPAR) /Ly6 domain
B cells, B cell-biased lymphoid progenitor (BLP), thymocytes, plasmacytoid dendritic cells
Cell-cell adhesion and signal transduction
Cell Type
B cells, Thymocytes, Dendritic cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules
Antigen References

1. Inlay MA, et al. 2009. Genes Dev. 23:2376.
2. Gumley TP, et al. 1995. Immunol. Cell Biol. 73:277.
3. Mansson R, et al. 2010. Blood 115:2601.
4. Reese JT, et al. 2001. Dev. Immunol. 8:107.

Gene ID
17068 View all products for this Gene ID
View information about Ly-6D on

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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