- RMM-1 (See other available formats)
- Regulatory Status
- Other Names
- Immunoglobulin M
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse Ig cocktail
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
(PubMed link indicates BioLegend citation)
- Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
- Product Citations
AB_10899576 (BioLegend Cat. No. 406517)
AB_2650930 (BioLegend Cat. No. 406532)
AB_2561444 (BioLegend Cat. No. 406518)
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
|Purified anti-mouse IgM||RMM-1||FC, ELISA|
|PE anti-mouse IgM||RMM-1||FC|
|Biotin anti-mouse IgM||RMM-1||ELISA, FC|
|FITC anti-mouse IgM||RMM-1||FC|
|APC anti-mouse IgM||RMM-1||FC|
|PerCP/Cyanine5.5 anti-mouse IgM||RMM-1||FC|
|APC/Cyanine7 anti-mouse IgM||RMM-1||FC|
|Brilliant Violet 421™ anti-mouse IgM||RMM-1||FC|
|PE/Cyanine7 anti-mouse IgM||RMM-1||FC|
|Alexa Fluor® 488 anti-mouse IgM||RMM-1||FC|
|Brilliant Violet 605™ anti-mouse IgM||RMM-1||FC|
|Alexa Fluor® 647 anti-mouse IgM||RMM-1||FC|
|Purified anti-mouse IgM (Maxpar® Ready)||RMM-1||FC, CyTOF®|
|PE/Dazzle™ 594 anti-mouse IgM||RMM-1||FC|
|Brilliant Violet 510™ anti-mouse IgM||RMM-1||FC|
|TotalSeq™-A0450 anti-mouse IgM||RMM-1||PG|
|Brilliant Violet 711™ anti-mouse IgM||RMM-1||FC|
|APC/Fire™ 750 anti-mouse IgM||RMM-1||FC|
|TotalSeq™-C0450 anti-mouse IgM||RMM-1||PG|
|PE/Cyanine5 anti-mouse IgM||RMM-1||FC|
|TotalSeq™-B0450 anti-mouse IgM||RMM-1||PG|
|Spark Violet™ 423 anti-mouse IgM||RMM-1||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse IgM
PE anti-mouse IgM
Biotin anti-mouse IgM
FITC anti-mouse IgM
APC anti-mouse IgM
PerCP/Cyanine5.5 anti-mouse IgM
APC/Cyanine7 anti-mouse IgM
Brilliant Violet 421™ anti-mouse IgM
PE/Cyanine7 anti-mouse IgM
Alexa Fluor® 488 anti-mouse IgM
Brilliant Violet 605™ anti-mouse IgM
Alexa Fluor® 647 anti-mouse IgM
Purified anti-mouse IgM (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse IgM
Brilliant Violet 510™ anti-mouse IgM
TotalSeq™-A0450 anti-mouse IgM
Brilliant Violet 711™ anti-mouse IgM
APC/Fire™ 750 anti-mouse IgM
TotalSeq™-C0450 anti-mouse IgM
PE/Cyanine5 anti-mouse IgM
TotalSeq™-B0450 anti-mouse IgM
Spark Violet™ 423 anti-mouse IgM