- MP5-32C11 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6). The MP5-32c11 antibody can not neutralize the bioactivity of natural or recombinant IL-6.Product Details
- Antibody Type
- Host Species
- COS-7-expressed, recombinant mouse IL-6
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5 - 2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1 - 4 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection1-4,8 or ELISPOT Detection5,6: The biotinylated MP5-32C11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified MP5-20F3 antibody (Cat. No. 504502/504506) as capture antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard.
Additional reported applications (for the relevant formats) include: Western blotting, In Vivo Capture7.
Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Sander B, et al. 1993. J. Immunol. Meth. 166:201.
- Starnes H, et al. 1990. J. Immunol. 145:4185.
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181.
- Mo X, et al. 1995. J. Virol. 69:1288.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
- O'Connell PJ, et al. 2006. Blood 107:1010.
- Product Citations
AB_2127458 (BioLegend Cat. No. 504601)
AB_2233898 (BioLegend Cat. No. 504602)
- Neuropoietic cytokine family; 22-29 kD (Mammalian)
- Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
- Cell Sources
- T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
- Cell Targets
- T cells, B cells, hepatocytes, cholinergic neurons
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.
- Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
- Gene ID
- 16193 View all products for this Gene ID
- View information about IL-6 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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