Biotin anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
a-N418_A_Biotin_Pos_071006
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and biotinylated N418 (top) or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE
  • a-N418_A_Biotin_Pos_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and biotinylated N418 (top) or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE
  • b-N418_B_Biotin_Neg_071006
  • c-N418_Biotin_CD11c_Antibody_3_012621
    Frozen mouse spleen sections. Endogenous biotin blocking applied prior to incubation of primary antibody anti‐mouse CD11c biotin followed by secondary detection with Alexa Fluor® 555 streptavidin. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Images provided by Ann Haberman and Tingting Zhang, Yale University.
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117303 50 µg 52€
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117304 500 µg 192€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Product Citations
  1. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  2. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  3. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  4. Hu G, et al. 2018. Immunity. 48:227. PubMed
  5. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  6. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  7. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  8. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  9. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  10. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  11. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  12. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  13. Kellermayer Z, et al. 2019. J Immunol. 202:1833. PubMed
  14. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  15. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  16. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  17. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  18. Stone MB et al. 2017. eLife. 6 pii: e19891. PubMed
  19. Yang Z et al. 2016. eLife. 5 pii: e21238. PubMed
  20. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  21. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  22. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  23. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  24. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  25. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  26. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  27. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  28. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  29. Liu S, et al. 2020. Neuron. S0896-6273(20)30532-8.. PubMed
  30. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  31. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  32. Jawhara S, et al. 2012. J Immunol. 189:2468. PubMed
  33. Palumbo R, et al. 2012. J Control Release. 159:232. PubMed
  34. Cheng Q, et al. 2014. J Immunol. 192:3686. PubMed
  35. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  36. Mori T, et al. 2014. J Immunol. 193:1728. PubMed
  37. Baptista A, et al. 2014. Elife. 3:4433. PubMed
  38. Lei G, et al. 2015. Infect Immun . 83:572. PubMed
  39. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  40. Hu Z, et al. 2015. J Exp Med. 101084/jm20150178. PubMed
  41. Spinner C, et al. 2015. Sci Rep. 5:16269. PubMed
  42. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
RRID
AB_313772 (BioLegend Cat. No. 117303)
AB_313773 (BioLegend Cat. No. 117304)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 4    Revision Date: 01/26/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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