APC anti-mouse Ly-6G Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Xue F, et al. 2020. Int J Biol Sci. 2.105555556. PubMed
2. Patras KA, et al. 2019. J Innate Immun. :1. PubMed
3. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
4. Li A, et al. 2020. Oncol Lett. 1.899305556. PubMed
5. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
6. Ding Z, et al. 2021. Int J Mol Sci. 22:. PubMed
7. Cornax I, et al. 2021. Microorganisms. 9:00. PubMed
8. Stein K, et al. 2016. J Leukoc Biol. 99: 231 - 239. PubMed
9. Christian D, et al. 2014. Infect Immun . 82:4056. PubMed
10. Sharma A, et al. 2014. J Infect Dis. . PubMed
11. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
12. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
13. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
14. Poli V, et al. 2021. iScience. 24:103256. PubMed
15. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
16. Weiss M, et al. 2015. Proc Natl Acad Sci U S A. 112: 11001-11006. PubMed
17. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
18. Gonçalves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
19. Kobayashi Y, et al. 2018. PLoS One. 13:e0195008. PubMed
20. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
21. Zhang Q, et al. 2021. Theranostics. 11:8301. PubMed
22. Han J, et al. 2022. FEBS J. 289:417. PubMed
23. Shani O, et al. 2020. Cancer Res. 80:5317. PubMed
24. Cheng G, et al. 2021. iScience. 24:102673. PubMed
25. Meng L, et al. 2021. Front Oncol. 11:691705. PubMed
26. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
27. Chen F, et al. 2022. Evid Based Complement Alternat Med. 2022:1240242. PubMed
28. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
29. Patnode M, et al. 2014. J Exp Med. 211:1281. PubMed
30. Liao H, et al. 2015. Rheumatology. 54: 1317 - 1326. PubMed
31. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
32. Thompson ER, et al. 2020. Am J Transplant. . PubMed
33. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
34. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
35. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
36. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
37. Bourn JR, et al. 2021. Cancer Lett. 503:75. PubMed
38. Yao ME, et al. 2022. Evid Based Complement Alternat Med. 2022:7784148. PubMed
39. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
40. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
41. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
42. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
43. Ma Y, et al. 2016. Cardiovasc Res. 110: 51 - 61. PubMed
44. Karmakar M, et al. 2016. Nat Commun. 7:10555. PubMed
45. Chu C, et al. 2016. Dis Model Mech. 9: 473-481. PubMed
46. Liu M, et al. 2020. Cell Rep. 32:107990. PubMed
47. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
48. Parlet CP, et al. 2019. Cell Rep. 27:187. PubMed
49. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
50. Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed
51. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
52. Goldman E, et al. 2021. Cells. 10:. PubMed
53. Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed
54. Pan YC, et al. 2021. Mol Ther Oncolytics. 20:175. PubMed
55. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
56. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
57. Michael BD, et al. 2020. Cell Reports. 32(11):108150. PubMed
58. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
59. Horn KJ, et al. 2022. Nat Commun. 13:3321. PubMed
60. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
61. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
62. Bao C, et al. 2021. Infect Immun. 89:. PubMed
63. Guo P, et al. 2021. J Immunol. 207:408. PubMed
64. Cui C, et al. 2021. Cell. 184(12):3163-3177.e21. PubMed
65. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
66. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
67. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
68. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
69. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
70. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
71. Gottfried-Blackmore A, et al. 2009. Proc Natl Acad Sci U S A. 106:20918. PubMed
72. Yang J, et al. 2022. Microbiome. 10:149. PubMed
73. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
74. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
75. , et al. 2021. Eur J Immunol. 51:2708. PubMed
76. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
77. Wang D, et al. 2021. Nat Commun. 12:2674. PubMed
78. Zhu H, et al. 2021. Cell Metabolism. :. PubMed
79. Tartey S, et al. 2021. Cancer Research. 81(9):2358-2372. PubMed
80. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
81. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
82. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
83. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
84. Wang SS et al. 2018. Cell chemical biology. 25(12):1519-1532 . PubMed
85. Cho K, et al. 2021. iScience. 24:103117. PubMed
86. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
87. Shan C, et al. 2021. Front Immunol. 12:766401. PubMed
88. Machácek T, et al. 2022. PLoS Pathog. 18:e1010302. PubMed
89. Xiao Y, et al. 2022. Dis Markers. 2022:1380560. PubMed
90. Bolkhovitina EL, et al. 2022. Int J Mol Sci. 23: . PubMed
91. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
92. Price P, et al. 2015. J Immunol. 194:1164. PubMed
93. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
94. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
95. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
96. Li H, et al. 2019. Front Immunol. 2.205555556. PubMed
97. Endoh M, et al. 2020. Cell Reports. 30(6):1823-1834. PubMed
98. Toth K, et al. 2022. PLoS Biol. 20:e3001526. PubMed
99. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
100. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
101. Steichen A, et al. 2015. PLoS One. 10:123573. PubMed
102. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
103. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
104. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
105. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
106. Janela B, et al. 2019. Immunity. 50:1069. PubMed
107. Smith CA, et al. 2019. JCI Insight. 4. PubMed
108. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
109. Namiguchi K, et al. 2022. Front Cardiovasc Med. 8:761591. PubMed
110. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
111. Patankar Y, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L902 - L913. PubMed
112. Ioannidis L, et al. 2016. J Immunol. 196: 1227 - 1238. PubMed
113. Wang SS, et al. 2021. Cell Chemical Biology. 28(5):699-710.e5. PubMed
114. Peñaloza HF, et al. 2018. Biochem Biophys Rep. 13:12. PubMed
115. Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed
116. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
117. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
118. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
119. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
120. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
121. Li YT, et al. 2019. Elife. 8:e47642. PubMed
122. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
123. Wingrove E et al. 2019. Cell reports. 27(4):1277-1292 . PubMed
124. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
125. Ohno-Urabe S, et al. 2018. J Am Heart Assoc. 7:e007389. PubMed
126. Resende M, et al. 2019. J Immunol. 203:2451. PubMed
127. Salvagno C, et al. 2019. Nat Cell Biol. 1.229861111. PubMed
128. Cá B, et al. 2019. Front Microbiol. 1.876388889. PubMed
129. Georgiadou A, et al. 2022. Elife. 11:. PubMed
130. Kwon MY, et al. 2021. J Leukoc Biol. 110:711. PubMed
131. Skokowa J, et al. 2022. Nat Commun. 13:2948. PubMed
132. Lin T, et al. 2021. PLoS Pathog. 17:e1009557. PubMed
133. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
134. Chua Y, et al. 2016. J Immunol. 197: 3771 - 3781. PubMed
135. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
136. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
137. Chen L, et al. 2021. Theranostics. 11:6668. PubMed
138. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
139. Shi J, et al. 2022. Front Pharmacol. 12:818326. PubMed
140. Gautam S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 319:L661. PubMed
141. Kamei A, et al. 2012. J Infect Dis. 207:39. PubMed
142. Gardner P, et al. 2015. Am J Pathol. 185:1324. PubMed
143. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
144. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
145. Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed
146. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
147. Molgora M, et al. 2020. Cell. 182:886. PubMed
148. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
149. Asahara M, et al. 2022. PLoS One. 17:e0276135. PubMed
150. Zhang XL, et al. 2022. J Am Heart Assoc. 11:e023800. PubMed
151. Andreou T, et al. 2021. J Immunother Cancer. 9:. PubMed
152. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
153. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
154. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
155. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
156. Heuslein J, et al. 2016. Sci Rep. 6: 27029. PubMed
157. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
158. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
159. Weng JH, et al. 2021. Nat Metab. 3:513. PubMed
160. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
161. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
162. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
163. Zhang J et al. 2019. Cell Rep. 27(9):2679-2689 . PubMed
164. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
165. Oertli M, et al. 2011. J Immunol. 187:3578. PubMed
166. Xu Z, et al. 2022. Cell Death Dis. 13:502. PubMed
167. Wang S, et al. 2022. Blood. 140:171. PubMed
168. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
169. Young K, et al. 2021. Cell Stem Cell. . PubMed
170. Cardoso F, et al. 2015. PLoS One. 10: 0131904. PubMed
171. Devi S, et al. 2013. J Exp Med. 210:2321. PubMed
172. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
173. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
174. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed

RRID

AB_1877163 (BioLegend Cat. No. 127613)
AB_2227348 (BioLegend Cat. No. 127614)

Antigen Details

Structure

A 21-35 kD GPI-anchorded membrane protein

Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type

Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID

546644 View all products for this Gene ID

UniProt

View information about Ly-6G on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis