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Alexa Fluor® 488 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_A488_CD3_Antibody_4_092121
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
Watch the video.
  • 17A2_A488_CD3_Antibody_4_092121
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
  • 17A2_Alexa488_071805
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 488
  • 17A2_Alexa488_CD3_IHC-F_120816
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL CD3 (Clone 17A2) Alexa Fluor® 488 (green) and B220 (Clone RA3-6B2) Brilliant Violet 421™ (blue), overnight at 4°C. The image was captured by 10X objective.
  • 3_Still-image_0316-5_20X-1_1
    Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD3 (clone 17A2) Alexa Fluor® 488 (red), CD21/35 (CR2/CR1)(clone 7E9) Alexa Fluor® 594 (green), and CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 (blue) all at 5 µg/mL, optically cleared, then analyzed at 215 μm imaging depth on a confocal microscope.
Compare all formats See Alexa Fluor® 488 spectral data See high resolution IHC-F data...
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100212 25 µg 81€
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100210 100 µg 184€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Schneider C, et al. 2018. Cell. 174:271. PubMed
  2. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  3. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  4. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  5. Bieber M, et al. 2021. Int J Mol Sci. 22:. PubMed
  6. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  7. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  8. Dauer Née Joppe K, et al. 2021. J Neurochem. 159:554. PubMed
  9. Upadhyaya P, et al. 2021. J Immunother Cancer. 9:00. PubMed
  10. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  11. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  12. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  13. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  14. Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed
  15. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  16. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  17. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  18. Lau CH, et al. 2022. Antioxidants (Basel). 11:. PubMed
  19. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  20. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
  21. Amor C, et al. 2020. Nature. 583:127. PubMed
  22. Wei SC, et al. 2020. Cancer Discov. . PubMed
  23. Mills C, et al. 2022. Cells. 11:. PubMed
  24. Wong HS, et al. 2021. Cell. . PubMed
  25. Xiao S, et al. 2007. J Exp Med. 204:1691. PubMed
  26. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  27. Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
  28. Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed
  29. Wang B, et al. 2021. J Immunother Cancer. 9:. PubMed
  30. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  31. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  32. Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed
  33. Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed
  34. Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed
  35. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  36. Langhauser F, et al. 2014. Stroke. 45:1799. PubMed
  37. Karandikar SH, et al. 2019. JCI Insight. 5. PubMed
  38. Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed
  39. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  40. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  41. Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed
  42. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  43. Zhao L,et al. 2017. Nat Commun. . 10.1038/s41467-017-01181-4. PubMed
  44. Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed
  45. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  46. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  47. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  48. Lee-Sayer SSM, et al. 2018. Eur J Immunol. 48:803. PubMed
  49. Zhou P, et al. 2022. Mol Ther Oncolytics. 25:236. PubMed
  50. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  51. Hsing CH, et al. 2021. Biomedicines. 9:. PubMed
RRID
AB_493530 (BioLegend Cat. No. 100212)
AB_389301 (BioLegend Cat. No. 100210)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 5    Revision Date: 09/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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