Ultra-LEAF™ Purified anti-mouse CD2 Antibody

Pricing & Availability
Clone
RM2-5 (See other available formats)
Regulatory Status
RUO
Other Names
LFA-2, T11, Ly-37, SRBC-R
Isotype
Rat IgG2b, λ
Ave. Rating
Submit a Review
Product Citations
publications
RM2-5_LEAF_021306.jpg
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified RM2-5, followed by anti-rat IgG FITC
  • RM2-5_LEAF_021306.jpg
    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified RM2-5, followed by anti-rat IgG FITC
Compare all formats
Cat # Size Price Quantity Check Availability Save
100118 100 µg 204 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100119 1 mg 660 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Block, Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
  2. Ayroldi E, et al. 1997. Blood 89:3717. (Block)
  3. Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
  4. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
RRID
AB_2832256 (BioLegend Cat. No. 100118)
AB_2832257 (BioLegend Cat. No. 100119)

Antigen Details

Structure
Ig superfamily, 45-58 kD
Distribution

T cells, thymocytes, NK cells, B cells

Function
T cell activation, adhesion
Ligand/Receptor
CD48, lck
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.

Gene ID
12481 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02.19.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD2

  • FITC anti-mouse CD2

  • PE anti-mouse CD2

  • Purified anti-mouse CD2

  • APC anti-mouse CD2

  • PE/Cyanine7 anti-mouse CD2

  • PerCP/Cyanine5.5 anti-mouse CD2

  • TotalSeq™-A0892 anti-mouse CD2

  • Ultra-LEAF™ Purified anti-mouse CD2

  • TotalSeq™-C0892 anti-mouse CD2

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account