Recombinant Human TGF-β3 (carrier-free)

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Other Names
Transforming growth factor beta 3 (TGF-b3), ARVD, TGFB3
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TGFB3_Human_Recombinant_Protein_BA_010813
TGF-β3 effect on HT-2 cell proliferation induced by mouse IL-4.
  • TGFB3_Human_Recombinant_Protein_BA_010813
    TGF-β3 effect on HT-2 cell proliferation induced by mouse IL-4.
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585802 5 µg 125 CHF
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Description

Human TGF-β3 precursor consists of two disulfide-linked identical subunits with 412 amino acids each. The carboxy-terminal (112 residues) of TGF-β3 represents the biologically active form of this protein; this portion of TGF-β3 exhibits 86/112 and 89/112 identical residues compared with TGF-β1 and TGF-β2, respectively. TGF-β3 precursor is cleaved by furin, and localized in the residue 300 of the trans-Golgi network. Furin processes the TGF-β3 precursor at the carboxyl side of the consensus sequence RKKR which precedes the NH2-terminal Ala 301 residue of the mature TGF-β3. The TGF-β3 precursor includes (in the N-terminal portion) the latency-associated peptide (LAP dimer) and (in the C-terminal) the 25 kD portion that constitutes the mature TGF-β3.

The LAP dimer and the TGF-β3 mature protein remain non-covalently associated after furin cleavage, and this complex does not bind to the TFG-β receptor. In addition, the TGF-β3 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active form requires dissociation from LAP. Some activators can release TGF-β1 from LAP, such as thrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8, and these activators might release TGF-β3 through similar mechanism. TGF-β3 induces biomechanical remodeling in avian embryonic atrioventricular valves. Also, gene delivery of a mutant TGF-β3 reduces re-epithelialization density and fibroblast/myofibroblast transdifferentiation within the wound area, both mechanisms are indicative of reduced scar tissue formation. In fact, recombinant TGF-β3 has been used in clinical trials as prophylactic treatment of human scars.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TGF-β3, amino acids Ala301-Ser412 (Accession# NM_003239.2) was expressed in CHO cells.
Molecular Mass
The 112 amino acid recombinant protein has a predicted molecular mass of approximately 12.7 kD. The DTT-reduced protein migrates at approximately 14 kD and non-reduced protein migrates at 28-30 kD by SDS-PAGE. The N-terminal amino acid is Alanine.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 25% Acetonitrile, 0.1% TFA (trifluoroacetic acid).
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
5 µg size is bottled at 100 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
TGF-β3 inhibits the proliferation of mouse HT-2 cells induced by IL-4. The ED50 is from 0.10 to 0.4 ng/ml, corresponding to a specific activity of 2.5-10 x 106 units/mg.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution
TGF-β3 is expressed in the upper layers of the epidermis, hair follicle, and sebaceous gland throughout the wound healing process. Also, it is expressed in unwounded tissues in the suprabasal layers of epidermis and in hair follicles.
Function
TGF-β3 participates in skin wound healing, is necessary in wound closure, decreases keratinocyte proliferation, and has anti-scaring effects. TGF-β3 is important in orofacial, lung, and atrioventricular valve development.
Interaction
Keratinocytes, fibroblasts, myofibroblasts, epithelial cells, lens epithelial cells. The majority of mammalian cells express the TGF-β receptors.
Ligand/Receptor
TGFBR1 and TGFBR2 receptors.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Embryonic Stem Cells
Biology Area
Cell Biology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Dubois CM, et al. 1995. J. Biol. Chem. 270:10618.
2. Ten Dijke P, et al. 1988. Proc. Natl. Acad. Sci. USA 85:4715.
3. Ferguson MW, et al. 2009. Lancet. 373:1264.
4. Waddington SN, et al. 2010. Mol. Ther. 18:2104.
5. Le M, et al. 2012. PLoS One 7:e48040.

Gene ID
7043 View all products for this Gene ID
UniProt
View information about TGF-beta3 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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