Purified anti-mouse CD105 Antibody

Pricing & Availability
Clone
MJ7/18 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_MJ7slash18_PURE_CD105_Antibody_IHC-F_Spleen_060817
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD105 (clone MJ7/18) and 10 µg/ml of Alexa Fluor® 647 anti-mouse CD3ε (clone 145-2C11) (green) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A_MJ7slash18_PURE_CD105_Antibody_IHC-F_Spleen_060817
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD105 (clone MJ7/18) and 10 µg/ml of Alexa Fluor® 647 anti-mouse CD3ε (clone 145-2C11) (green) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • B_MJ7slash18_PURE_CD105_Antibody_IHC-F_Liver_060817
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD105 (clone MJ7/18) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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120401 50 µg 66 CHF
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120402 500 µg 242 CHF
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Description

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Inflamed mouse skin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
WB, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Ge AZ and Butcher EC. 1994. Gene 138:201.
  2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
  3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
  4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)
Product Citations
  1. Cao Y et al. 2018. Molecular metabolism. 14:71-81 . PubMed
  2. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  3. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  4. Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed
  5. Stritt S, et al. 2016. Nat Commun. 7:11097. PubMed
  6. Lehmann ML, et al. 2020. Brain Behav Immun. 88:735. PubMed
RRID
AB_961066 (BioLegend Cat. No. 120401)
AB_961070 (BioLegend Cat. No. 120402)

Antigen Details

Structure
Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution

Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast

Function
Suppresses TGF-β signaling, angiogenesis
Ligand/Receptor
TGF-β1, TGF-β3
Cell Type
Endothelial cells, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.

Gene ID
13805 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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