- 11C3C65 (See other available formats)
- Regulatory Status
- Other Names
- CD223, LAG-3, LAG3, lymphocyte-activation gene-3
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|369302||100 µg||270 CHF|
CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human LAG-3 transfected cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
- Product Citations
AB_2616876 (BioLegend Cat. No. 369302)
- 70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Activated T-cells and natural killer cells (NKs) and regulatory T cells.
- Negatively regulates T-cell activation.
- Binds MHC class II molecules.
- Cell Type
- Dendritic cells, NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology. 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.
- Gene ID
- 3902 View all products for this Gene ID
- View information about CD223 on UniProt.org
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD223 (LAG-3)
Alexa Fluor® 647 anti-human CD223 (LAG-3)
PE anti-human CD223 (LAG-3)
FITC anti-human CD223 (LAG-3)
PE/Cyanine7 anti-human CD223 (LAG-3)
PerCP/Cyanine5.5 anti-human CD223 (LAG-3)
Brilliant Violet 421™ anti-human CD223 (LAG-3)
Brilliant Violet 650™ anti-human CD223 (LAG-3)
Brilliant Violet 510™ anti-human CD223 (LAG-3)
Brilliant Violet 785™ anti-human CD223 (LAG-3)
Brilliant Violet 711™ anti-human CD223 (LAG-3)
Brilliant Violet 605™ anti-human CD223 (LAG-3)
Alexa Fluor® 488 anti-human CD223 (LAG-3)
Biotin anti-human CD223 (LAG-3)
PE/Dazzle™ 594 anti-human CD223 (LAG-3)
APC/Fire™ 750 anti-human CD223 (LAG-3)
TotalSeq™-A0152 anti-human CD223 (LAG-3)
TotalSeq™-C0152 anti-human CD223 (LAG-3)
TotalSeq™-B0152 anti-human CD223 (LAG-3)
TotalSeq™-D0152 anti-human CD223 (LAG-3)
Alexa Fluor® 700 anti-human CD223 (LAG-3)
Pacific Blue™ anti-human CD223 (LAG-3)
PE/Cyanine5 anti-human CD223 (LAG-3)
APC/Cyanine7 anti-human CD223 (LAG-3)
APC/Fire™ 810 anti-human CD223 (LAG-3)