PerCP/Cyanine5.5 anti-human CD20, CD20, 2H7

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: 2H7
Workshop: IV B201
Other Names: B1, Bp35
Isotype: Mouse IgG2b, κ

2H7_PerCPCyanine55_CD20_ASR_111519 — Typical results from human peripheral blood lymphocytes stained either with 2H7 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

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Size

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980210

500 µL

576 CHF

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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

50 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. Do not freeze.

Application

Suggested for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
Do not use this reagent beyond the expiration date stated on the label.
Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References

Hultin L, et al. 1993. Cytometry 14:196.
Tedder T, et al. 1994. Immunol. Today 15:450.

Documentation

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07.08.2020

Analyte Specific Reagent. Analytical and performance characteristic are not established.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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