PE/Fire™ 640 anti-human CD45 Antibody, CD45, HI30

Pricing & Availability

Clone: HI30 (See other available formats)
Regulatory Status: RUO
Workshop: IV N816
Other Names: LCA, T200
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

Human peripheral blood lymphocytes were stained with CD45 (clone HI30) PE/Fire™ 640 (filled histogram) or unstained control (open histogram).

Compare all formats See PE/Fire™ 640 spectral data

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Size

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304073

25 tests

176 CHF

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304074

100 tests

382 CHF

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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹, Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Kishihara K, et al. 1993. Cell 74:143.
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
Nagano M, et al. 2007. Blood 110:151.
Jiang Q, et al. 2008. Blood 112:2858. PubMed
Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
Lee J, et al. 2015. J Exp Med. 212:385. PubMed
Breton G, et al. 2015. J Exp Med. 212:401. PubMed
Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2860789 (BioLegend Cat. No. 304073)
AB_2860790 (BioLegend Cat. No. 304074)

Antigen Details

Structure

Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)

Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function

TCR and BCR mediated activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Cell Type

Hematopoietic stem and progenitors, Mesenchymal Stem Cells

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

Thomas M. 1989. Annu. Rev. Immunol. 7:339.
Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID

5788 View all products for this Gene ID

UniProt

View information about CD45 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

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Brilliant Violet 421™ anti-human CD45	HI30	FC
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Brilliant Violet 510™ anti-human CD45	HI30	FC
Brilliant Violet 605™ anti-human CD45	HI30	FC
Brilliant Violet 650™ anti-human CD45	HI30	FC
Purified anti-human CD45 (Maxpar® Ready)	HI30	FC,CyTOF®
Brilliant Violet 785™ anti-human CD45	HI30	FC
Brilliant Violet 711™ anti-human CD45	HI30	FC
PE/Dazzle™ 594 anti-human CD45	HI30	FC
Alexa Fluor® 594 anti-human CD45	HI30	IHC-P,SB
APC/Fire™ 750 anti-human CD45	HI30	FC
Pacific Blue™ anti-human CD45	HI30	FC
APC anti-human CD45	HI30	FC
PE/Cyanine7 anti-human CD45	HI30	FC
PE/Dazzle™ 594 anti-human CD45	HI30	FC
TotalSeq™-A0391 anti-human CD45	HI30	PG
TotalSeq™-B0391 anti-human CD45	HI30	PG
TotalSeq™-C0391 anti-human CD45	HI30	PG
PerCP/Cyanine5.5 anti-human CD45	HI30	FC
APC/Fire™ 750 anti-human CD45	HI30	FC
PE/Fire™ 640 anti-human CD45	HI30	FC
APC/Fire™ 810 anti-human CD45	HI30	FC
Spark YG™ 570 anti-human CD45	HI30	IHC-P
PE/Fire™ 700 anti-human CD45	HI30	FC
FITC anti-human CD45	HI30	FC
PerCP anti-human CD45	HI30	FC
Alexa Fluor® 660 anti-human CD45 Antibody	HI30	FC
Spark Violet™ 538 anti-human CD45	HI30	FC
Spark YG™ 593 anti-human CD45	HI30	FC
GMP APC/Fire™ 750 anti-human CD45	HI30	FC
Spark Violet™ 538 anti-human CD45	HI30	FC
GMP APC anti-human CD45	HI30	FC
Spark UV™ 387 anti-human CD45	HI30	FC
GMP Pacific Blue™ anti-human CD45	HI30	FC
GMP PerCP anti-human CD45	HI30	FC
GMP FITC anti-human CD45	HI30	FC
PE anti-human CD45	HI30	FC
GMP PE/Dazzle™ 594 anti-human CD45	HI30	FC
GMP PerCP/Cyanine5.5 anti-human CD45	HI30	FC
Spark Blue™ 515 anti-human CD45	HI30	FC
TotalSeq™-D0391 anti-human CD45	HI30	PG
GMP PE/Cyanine7 anti-human CD45	HI30	FC
Spark Violet™ 500 anti-human CD45	HI30	FC
GMP PE anti-human CD45	HI30	FC
Spark PLUS UV™ 395 anti-human CD45	HI30	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 08.28.2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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