PE/Cyanine7 anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMM-1_PECy7_1_090310
C57BL/6 splenocytes stained with CD19 (6D5) APC and RMM-1 PE/Cyanine7
  • RMM-1_PECy7_1_090310
    C57BL/6 splenocytes stained with CD19 (6D5) APC and RMM-1 PE/Cyanine7
  • RMM-1_PECy7_2_090310
    C57BL/6 splenocytes stained with CD19 (6D5) APC and rat IgG2a PE/Cyanine7 isotype control
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
406513 25 µg 75 CHF
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406514 100 µg 195 CHF
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  2. Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed
  3. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  4. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  5. Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed
  6. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  7. Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed
  8. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  9. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  10. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
RRID
AB_10640069 (BioLegend Cat. No. 406513)
AB_10642031 (BioLegend Cat. No. 406514)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01.29.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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