PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RA3-6B2_PECy7_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
  • RA3-6B2_PECy7_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
103221 25 µg 80 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103222 100 µg 180 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. El-Zaatari M, et al. 2017. Gastroenterology. 10.1053/j.gastro.2017.09.002. PubMed
  2. Sakamoto A, et al. 2017. Int Immunol. . 10.1093/intimm/dxx054. PubMed
  3. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  4. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  5. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  6. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  7. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  8. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  9. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  10. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  11. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  12. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  13. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  14. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  15. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  16. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  17. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  18. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  19. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  20. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  21. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  22. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  23. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  24. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  25. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  26. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  27. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  28. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  29. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  30. Lamoureux J, et al. 2007. J Exp Med. 204:2853. PubMed
  31. Aït-Azzouzene D, et al. 2010. J Immunol. 185:1015. PubMed
  32. Stacey M, et al. 2011. J Immunol. 187:2944. PubMed
  33. Ouchida R, et al. 2012. Proc Natl Acad Sci U S A. 109:e2699. PubMed
  34. Heizmann B, et al. 2013. J Exp Med. 210:2823. PubMed
  35. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  36. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  37. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  38. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  39. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  40. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  41. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  42. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  43. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  44. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  45. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  46. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  47. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  48. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  49. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  50. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  51. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  52. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  53. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  54. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  55. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  56. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
RRID
AB_313004 (BioLegend Cat. No. 103221)
AB_313005 (BioLegend Cat. No. 103222)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.25.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account