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PE/Cyanine7 anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12_PECy7_2_102110
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE/Cyanine7 (filled histogram) or rat IgG2a PE/Cyanine7 isotype control (open histogram) (gated on lymphoid cell population).
  • TC15-12F12_PECy7_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE/Cyanine7 (filled histogram) or rat IgG2a PE/Cyanine7 isotype control (open histogram) (gated on lymphoid cell population).
  • TC15-12F12dot2_PECy7_957
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE/Cyanine7 (filled histogram) or rat IgG2a PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
115913 25 µg 112 CHF
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115914 100 µg 311 CHF
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  2. Upadhaya S, et al. 2020. Cell Stem Cell. 27(2):336-345.e4. PubMed
  3. Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
  4. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  6. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  7. Muto T, et al. 2020. Nat Immunol. 1.246527778. PubMed
  8. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  9. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  10. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  11. Grinenko T, et al. 2018. Nat Commun. 9:1898. PubMed
  12. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  13. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  14. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  15. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  16. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  17. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  18. Wilkinson AC, et al. 2020. Nature Protocols. 15(2):628-648.. PubMed
  19. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  20. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  21. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  22. Jordan M, et al. 2011. J Immunol. 186:3953. PubMed
  23. Eisele AS, et al. 2022. Elife. 11:. PubMed
  24. Gustafsson K, et al. 2013. Exp Cell Res. 319:1852. PubMed
  25. Halene S, et al. 2010. Blood. 116:1942. PubMed
  26. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  27. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  28. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  29. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  30. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  31. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  32. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
  33. Zhong L, et al. 2022. JCI Insight. 7:. PubMed
  34. Clapes T, et al. 2021. Nat Cell Biol. 23:704. PubMed
  35. Yankova E, et al. 2021. Nature. 593:597. PubMed
  36. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  37. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  38. Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
  39. Maetzig T, et al. 2017. Mol Ther. 25:606. PubMed
  40. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  41. Tober J, et al. 2013. Development. 140:3765. PubMed
  42. Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
  43. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  44. Liang R, et al. 2020. Cell Stem Cell. 359:26. PubMed
  45. Yu W, et al. 2020. J Clin Invest. . PubMed
  46. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  47. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  48. Karamitros D, et al. 2015. Development. 142:70. PubMed
  49. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  50. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  51. Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed
  52. Xia P, et al. 2015. J Exp Med. 212: 2305 - 2321. PubMed
  53. Nestorowa S, et al. 2016. Blood. 128: e20 - e31. PubMed
  54. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  55. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  56. Glotzbach J, et al. 2011. PLoS One. 6:e21211. PubMed
  57. Young IC, et al. 2021. Cell Rep. 37:110036. PubMed
  58. Koide S, et al. 2022. iScience. 25:103603. PubMed
  59. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  60. Ueda K, et al. 2021. Cancer Cell. 39(4):529-547.e7. PubMed
  61. Chlon TM, et al. 2021. Cell Stem Cell. :. PubMed
  62. Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed
  63. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
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  65. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
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  67. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  68. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  69. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  70. Guo F, et al. 2013. Haematologica. 98:1353. PubMed
  71. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  72. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  73. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  74. Fast EM, et al. 2021. Elife. 10:. PubMed
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RRID
AB_439796 (BioLegend Cat. No. 115913)
AB_439797 (BioLegend Cat. No. 115914)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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